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(-) Description

Title :  PRE-CLEAVAGE STATE OF THE THERMOANAEROBACTER TENGCONGENSIS GLMS RIBOZYME BOUND TO GLUCOSE-6-PHOSPHATE
 
Authors :  D. J. Klein, A. R. Ferre-D'Amare
Date :  15 May 06  (Deposition) - 26 Sep 06  (Release) - 24 Feb 09  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.70
Chains :  Asym./Biol. Unit :  A,B
Keywords :  Rna, Ribozyme, Pseudoknot, Helix (Keyword Search: [Gene Ontology, PubMed, Web (Google))
 
Reference :  D. J. Klein, A. R. Ferre-D'Amare
Structural Basis Of Glms Ribozyme Activation By Glucosamine-6-Phosphate
Science V. 313 1752 2006
PubMed-ID: 16990543  |  Reference-DOI: 10.1126/SCIENCE.1129666
(for further references see the PDB file header)

(-) Compounds

Molecule 1 - GLMS RIBOZYME DEOXY RNA INHIBITOR
    ChainsA
    EngineeredYES
    Other DetailsADENOSINE 0 IS DEOXY, ITS O2* IS NOT MISSING
    Other Details - SourceTHIS SEQUENCE OCCURS NATURALLY IN THERMOANAEROBACTER TENGCONGENSIS
    SyntheticYES
 
Molecule 2 - GLMS RIBOZYME RNA
    ChainsB
    EngineeredYES
    Other DetailsRNA PRODUCED BY IN VITRO TRANSCRIPTION. THIS SEQUENCE OCCURS NATURALLY IN THERMOANAEROBACTER TENGCONGENSIS
    SyntheticYES

 Structural Features

(-) Chains, Units

  12
Asymmetric/Biological Unit AB

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (2, 9)

Asymmetric/Biological Unit (2, 9)
No.NameCountTypeFull Name
1G6P1Ligand/IonALPHA-D-GLUCOSE-6-PHOSPHATE
2MG8Ligand/IonMAGNESIUM ION

(-) Sites  (8, 8)

Asymmetric Unit (8, 8)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREG A:1 , C A:2 , HOH A:204 , A B:35 , A B:50 , U B:51 , G B:65 , G B:66 , MG B:406BINDING SITE FOR RESIDUE G6P A 301
2AC2SOFTWAREG A:1 , C A:2 , A B:35 , C B:36 , G B:37 , HOH B:201 , HOH B:202BINDING SITE FOR RESIDUE MG A 401
3AC3SOFTWAREA B:56 , C B:57 , U B:59BINDING SITE FOR RESIDUE MG B 402
4AC4SOFTWAREU B:79 , C B:81 , C B:82BINDING SITE FOR RESIDUE MG B 403
5AC5SOFTWAREU B:100 , G B:101BINDING SITE FOR RESIDUE MG B 404
6AC6SOFTWAREG A:3 , G B:122BINDING SITE FOR RESIDUE MG B 405
7AC7SOFTWAREG6P A:301 , G B:64BINDING SITE FOR RESIDUE MG B 406
8AC8SOFTWAREG B:86 , G B:139BINDING SITE FOR RESIDUE MG B 407

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 2H0Z)

(-) Cis Peptide Bonds  (0, 0)

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 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

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(-) Exons   (0, 0)

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(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
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SAPs(SNPs) PROSITE motifs Exons
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Chain A from PDB  Type:DNA/RNA  Length:21
                                                     
                 2h0z A   0 AGCGCCUGGACUUAAAGCCAU  20
                                     9        19 

Chain B from PDB  Type:RNA  Length:123
                                                                                                                                                           
                 2h0z B  23 GGCUUUAAGUUGACGAGGGCAGGGUUUAUCGAGACAUCGGCGGGUGCCCUGCGGUCUUCCUGCGACCGUUAGAGGACUGGUAAAACCACAGGCGACUGUGGCAUAGAGCAGUCCGGGCAGGAA 145
                                    32        42        52        62        72        82        92       102       112       122       132       142   

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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

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(-) Pfam Domains  (0, 0)

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(-) Gene Ontology  (0, 0)

Asymmetric/Biological Unit(hide GO term definitions)
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(-) Related Entries Specified in the PDB File

2gcs 2gcv 2h0s 2h0w 2h0x