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(-) Description

Title :  NMR SOLUTION STRUCTURE OF THE HYPER-SPORULATION RESPONSE REGULATOR SPO0F MUTANT I90A FROM BACILLUS SUBTILIS
 
Authors :  B. G. Bobay, P. D. Mclaughlin, R. J. Thompson, J. A. Hoch, J. Cavanagh
Date :  20 Sep 07  (Deposition) - 05 Feb 08  (Release) - 24 Feb 09  (Revision)
Method :  SOLUTION NMR
Resolution :  NOT APPLICABLE
Chains :  NMR Structure  :  A  (10x)
Keywords :  Response Regulator, Two-Component Systems, Bacterial Signal Transduction, Phospho-Relay, (Beta/Alpha)5 Protein, Cytoplasm, Kinase, Magnesium, Metal-Binding, Phosphorylation, Sporulation, Transferase, Two-Component Regulatory System (Keyword Search: [Gene Ontology, PubMed, Web (Google))
 
Reference :  B. G. Bobay, R. A. White, H. Szurmant, D. M. Sullivan, P. D. Mclaughlin, T. Hwa, J. A. Hoch, J. Cavanagh
Covariance Identifies Dynamic Residues At The Interfaces In Protein/Protein Complexes
To Be Published
PubMed: search
(for further references see the PDB file header)

(-) Compounds

Molecule 1 - SPORULATION INITIATION PHOSPHOTRANSFERASE F
    ChainsA
    EC Number2.7.-.-
    EngineeredYES
    Expression SystemESCHERICHIA COLI BL21(DE3)
    Expression System PlasmidPET-20B
    Expression System StrainBL21(DE)3
    Expression System Taxid469008
    Expression System Vector TypePLASMID
    GeneSPO0F
    MutationYES
    Organism ScientificBACILLUS SUBTILIS
    Organism Taxid1423
    SynonymSTAGE 0 SPORULATION PROTEIN F

 Structural Features

(-) Chains, Units

  
NMR Structure (10x)

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (0, 0)

(no "Ligand,Modified Residues,Ions" information available for 2JVJ)

(-) Sites  (0, 0)

(no "Site" information available for 2JVJ)

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 2JVJ)

(-) Cis Peptide Bonds  (0, 0)

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 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 2JVJ)

(-) PROSITE Motifs  (1, 1)

NMR Structure (1, 1)
 PROSITEUniProtKBPDB
No.IDACDescriptionIDLocationCountLocation
1RESPONSE_REGULATORYPS50110 Response regulatory domain profile.SP0F_BACSU5-119  1A:5-119

(-) Exons   (0, 0)

(no "Exon" information available for 2JVJ)

(-) Sequences/Alignments

NMR Structure
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
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Chain A from PDB  Type:PROTEIN  Length:132
 aligned with SP0F_BACSU | P06628 from UniProtKB/Swiss-Prot  Length:124

    Alignment length:132
                                                                                                                                                     124        
                                    10        20        30        40        50        60        70        80        90       100       110       120   |     -  
           SP0F_BACSU     1 MMNEKILIVDDQYGIRILLNEVFNKEGYQTFQAANGLQALDIVTKERPDLVLLDMKIPGMDGIEILKRMKVIDENIRVIIMTAYGELDMIQESKELGALTHFAKPFDIDEIRDAVKKYLPLKSN--------   -
               SCOP domains d2jvja_ A: Sporulation response regulator Spo0F                                                                                      SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------ CATH domains
               Pfam domains -----Response_reg-2jvjA01 A:6-116                                                                                   ---------------- Pfam domains
         Sec.struct. author ....eeeee....hhhhhhhhhhh....eeeee.hhhhhhhhhhhh...eeee.......hhhhhhhhhhhhh...eeee..hhhhhhhhhhhh............hhhhhhhhhhhhh............. Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------ SAPs(SNPs)
                    PROSITE ----RESPONSE_REGULATORY  PDB: A:5-119 UniProt: 5-119                                                                   ------------- PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------ Transcript
                 2jvj A   1 MMNEKILIVDDQYGIRILLNEVFNKEGYQTFQAANGLQALDIVTKERPDLVLLDMKIPGMDGIEILKRMKVIDENIRVIIMTAYGELDMAQESKELGALTHFAKPFDIDEIRDAVKKYLPLKSNLEHHHHHH 132
                                    10        20        30        40        50        60        70        80        90       100       110       120       130  

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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (1, 1)

NMR Structure

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 2JVJ)

(-) Pfam Domains  (1, 1)

NMR Structure
(-)
Clan: CheY (97)

(-) Gene Ontology  (8, 8)

NMR Structure(hide GO term definitions)
Chain A   (SP0F_BACSU | P06628)
molecular function
    GO:0016301    kinase activity    Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
    GO:0046872    metal ion binding    Interacting selectively and non-covalently with any metal ion.
    GO:0005515    protein binding    Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
    GO:0016740    transferase activity    Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
biological process
    GO:0000160    phosphorelay signal transduction system    A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
    GO:0016310    phosphorylation    The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
    GO:0030435    sporulation resulting in formation of a cellular spore    The process in which a relatively unspecialized cell acquires the specialized features of a cellular spore, a cell form that can be used for dissemination, for survival of adverse conditions because of its heat and dessication resistance, and/or for reproduction.
cellular component
    GO:0005737    cytoplasm    All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        SP0F_BACSU | P066281f51 1fsp 1nat 1pey 1pux 1srr 2fsp 2ftk 2jvi 2jvk 3q15

(-) Related Entries Specified in the PDB File

1fsp WILD-TYPE SPO0F
2jvi
2jvk