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(-) Description

Title :  RAT HEME OXYGENASE-1 IN COMPLEX WITH HEME AND CO WITH 1 HR ILLUMINATION: LASER ON
 
Authors :  M. Sugishima, K. Moffat, M. Noguchi
Date :  20 Jul 12  (Deposition) - 31 Oct 12  (Release) - 31 Oct 12  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.90
Chains :  Asym./Biol. Unit :  A
Keywords :  All Alpha Protein, Oxygenase, Oxidoreductase (Keyword Search: [Gene Ontology, PubMed, Web (Google))
 
Reference :  M. Sugishima, K. Moffat, M. Noguchi
Discrimination Between Co And O2 In Heme Oxygenase: Comparison Of Static Structures And Dynamic Conformation Changes Following Co Photolysis
Biochemistry 2012
PubMed-ID: 23043644  |  Reference-DOI: 10.1021/BI301175X

(-) Compounds

Molecule 1 - HEME OXYGENASE 1
    ChainsA
    EC Number1.14.99.3
    EngineeredYES
    Expression SystemESCHERICHIA COLI
    Expression System PlasmidPET-21A
    Expression System StrainBL21(DE3)
    Expression System Taxid562
    Expression System Vector TypePLASMID
    FragmentUNP RESIDUES 1-267
    GeneHMOX1
    Organism CommonRAT
    Organism ScientificRATTUS NORVEGICUS
    Organism Taxid10116
    SynonymHO-1, HSP32

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (3, 3)

Asymmetric/Biological Unit (3, 3)
No.NameCountTypeFull Name
1CMO1Ligand/IonCARBON MONOXIDE
2FMT1Ligand/IonFORMIC ACID
3HEM1Ligand/IonPROTOPORPHYRIN IX CONTAINING FE

(-) Sites  (3, 3)

Asymmetric Unit (3, 3)
No.NameEvidenceResiduesDescription
1AC1SOFTWARELYS A:18 , HIS A:25 , MET A:34 , GLN A:38 , TYR A:134 , THR A:135 , ARG A:136 , GLY A:139 , SER A:142 , GLY A:143 , ARG A:183 , PHE A:207 , ASN A:210 , PHE A:214 , CMO A:303 , HOH A:517BINDING SITE FOR RESIDUE HEM A 301
2AC2SOFTWAREARG A:27 , ARG A:185 , LEU A:208BINDING SITE FOR RESIDUE FMT A 302
3AC3SOFTWAREGLY A:139 , GLY A:143 , GLY A:144 , HEM A:301 , HOH A:432BINDING SITE FOR RESIDUE CMO A 303

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4G7T)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4G7T)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4G7T)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4G7T)

(-) Exons   (0, 0)

(no "Exon" information available for 4G7T)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
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SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it)
Chain A from PDB  Type:PROTEIN  Length:212
                                                                                                                                                                                                                                                    
               SCOP domains d4g7ta_ A: Heme oxygenase-1 (HO-1)                                                                                                                                                                                   SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .hhhhhhhhhhhhhhhhhhhhhhhhhhhh...hhhhhhhhhhhhhhhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhhhhhh.hhhhhh..hhhhhhhhhhhhhhhhhh..hhhhhhhhhhhhhhhhhhhhhhhhhhhhh.......hhhhh.....hhhhhhhhhhhhhh....hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4g7t A  11 QDLSEALKEATKEVHIRAENSEFMRNFQKGQVSREGFKLVMASLYHIYTALEEEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQALLT 222
                                    20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220  

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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (1, 1)

Asymmetric/Biological Unit

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4G7T)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4G7T)

(-) Gene Ontology  (64, 64)

Asymmetric/Biological Unit(hide GO term definitions)

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        HMOX1_RAT | P067621dve 1dvg 1irm 1ivj 1ix3 1ix4 1j02 1j2c 1ubb 1ulx 1vgi 2dy5 2e7e 2zvu 3i9t 3i9u 3wkt 4g7l 4g7p 4g7u 4g8p 4g8u 4g8w 4g98 4g99 4mec

(-) Related Entries Specified in the PDB File

4g7l 4g7p 4g7u 4g8p 4g8u 4g8w 4g98 4g99