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(-) Description

Title :  TUBR BOUND TO TUBC - 26 BP - FROM BACILLUS THURINGIENSIS SEROVAR ISRAELENSIS PBTOXIS
 
Authors :  C. H. S. Aylett, J. Lowe
Date :  02 May 12  (Deposition) - 03 Oct 12  (Release) - 24 Oct 12  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  7.00
Chains :  Asym./Biol. Unit :  A,B,C,D,E,F,G,H,I,Y,Z
Keywords :  Structural Protein-Dna Complex, Segregation, Partitioning (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  C. H. S. Aylett, J. Lowe
Superstructure Of The Centromeric Complex Of Tubzrc Plasmid Partitioning Systems.
Proc. Natl. Acad. Sci. Usa V. 109 16522 2012
PubMed-ID: 23010931  |  Reference-DOI: 10.1073/PNAS.1210899109

(-) Compounds

Molecule 1 - TUBR FROM BACILLUS THURINGIENSIS PBTOXIS
    Atcc: 35646
    Chains: A, B, C, D, E, F, G, H, I
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PHIS17
    Expression System Strain: BL21
    Expression System Taxid: 511693
    Expression System Variant: C41
    Expression System Vector Type: PLASMID
    Organism Scientific: BACILLUS THURINGIENSIS
    Organism Taxid: 1428
    Strain: SEROVAR ISRAELENSIS
 
Molecule 2 - TUBC FROM BACILLUS THURINGIENSIS PBTOXIS 26 BP
    Atcc: 35646
    Chains: Y
    Fragment: SENSE STRAND
    Organism Scientific: BACILLUS THURINGIENSIS
    Organism Taxid: 1428
    Strain: SEROVAR ISRAELENSIS
    Synthetic: YES
 
Molecule 3 - TUBC FROM BACILLUS THURINGIENSIS PBTOXIS 26 BP
    Atcc: 35646
    Chains: Z
    Fragment: ANTISENSE STRAND
    Organism Scientific: BACILLUS THURINGIENSIS
    Organism Taxid: 1428
    Strain: SEROVAR ISRAELENSIS
    Synthetic: YES

 Structural Features

(-) Chains, Units

  1234567891011
Asymmetric/Biological Unit : ABCDEFGHIYZ

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 27)

Asymmetric/Biological Unit (1, 27)
No.NameCountTypeFull Name
1MSE27Mod. Amino AcidSELENOMETHIONINE

(-) Sites  (0, 0)

(no "Site" information available for 4ASS)

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4ASS)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4ASS)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4ASS)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4ASS)

(-) Exons   (0, 0)

(no "Exon" information available for 4ASS)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass A  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain B from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass B  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain C from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass C  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain D from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass D  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain E from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass E  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain F from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass F  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain G from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass G  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain H from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .eeehhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass H  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain I from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ....hhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  4ass I  6 FYTLNIAEIAERIGNDDCAYQVLmAFINENGEAQmLNKTAVAEmIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25   |    35    |   45   |    55        65        75        85        95   
                                                  29-MSE     40-MSE   49-MSE                                             


Chain Y from PDB  Type:DNA  Length:26
                                                         
                  4ass Y  1 CTTTAAGTTTAACTTTCAGTTTACAT 26
                                    10        20      


Chain Z from PDB  Type:DNA  Length:26
                                                         
                  4ass Z  1 ATGTAAACTGAAAGTTAAACTTAAAG 26
                                    10        20
   Legend:   → Mismatch (orange background)
  - → Gap (green background, '-', border residues have a numbering label)
    → Modified Residue (blue background, lower-case, 'x' indicates undefined single-letter code, labelled with number + name)
  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4ASS)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4ASS)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4ASS)

(-) Gene Ontology  (1, 1)

Asymmetric/Biological Unit(hide GO term definitions)
Chain A,B,C,D,E,F,G,H,I   (Q8KNP2_BACTI | Q8KNP2)
molecular function
    GO:0003677    DNA binding    Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/TrEMBL
        Q8KNP2_BACTI | Q8KNP2: 3m8e 3m8f 3m9a 4aso

(-) Related Entries Specified in the PDB File

4asn TUBR FROM BACILLUS MEGATERIUM PBM400
4aso TUBR BOUND TO 24 BP OF TUBC FROM BACILLUS THURINGIENSIS SEROVAR ISRAELENSIS PBTOXIS