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(-) Description

Title :  AN X-RAY STRUCTURE OF A PUTATIVE PHOSPHOGYLCERATE KINASE WITH BOUND ADP FROM FRANCISELLA TULARENSIS SUBSP. TULARENSIS SCHU S4
 
Authors :  J. S. Brunzelle, Z. Wawrzak, T. Skarina, W. F. Anderson, A. Savchenko, C Structural Genomics Of Infectious Diseases (Csgid)
Date :  30 May 12  (Deposition) - 13 Jun 12  (Release) - 13 Jun 12  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.30
Chains :  Asym./Biol. Unit :  A
Keywords :  Structural Genomics, Niaid, National Institute Of Allergy And Infectious Diseases, Center For Structural Genomics Of Infectious Diseases, Csgid, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  J. S. Brunzelle, Z. Wawrzak, T. Skarina, W. F. Anderson, A. Savchenko, Center For Structural Genomics Of Infectious Diseases
An X-Ray Structure Of A Putative Phosphogylcerate Kinase With Bound Adp From Francisella Tularensis Subsp. Tularensi Schu S4
To Be Published
PubMed: search

(-) Compounds

Molecule 1 - PHOSPHOGLYCERATE KINASE
    Chains: A
    EC Number: 2.7.2.3
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PMCSG7
    Expression System Strain: BL21 DE3 GOLD MAGIC
    Expression System Taxid: 469008
    Expression System Vector Type: PLASMID
    Gene: FTT_1367C, PGK
    Organism Scientific: FRANCISELLA TULARENSIS SUBSP. TULARENSIS
    Organism Taxid: 177416
    Strain: SCHU S4 / SCHU 4

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit : A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 1)

Asymmetric/Biological Unit (1, 1)
No.NameCountTypeFull Name
1ADP1Ligand/IonADENOSINE-5'-DIPHOSPHATE

(-) Sites  (1, 1)

Asymmetric Unit (1, 1)
No.NameEvidenceResiduesDescription
1AC1SOFTWARELYS A:120 , GLY A:190 , GLY A:191 , SER A:192 , LYS A:197 , GLY A:215 , LEU A:233 , ILE A:288 , GLY A:316 , VAL A:317 , GLU A:319 , GLY A:346 , ASP A:347 , HOH A:538 , HOH A:548 , HOH A:565 , HOH A:593BINDING SITE FOR RESIDUE ADP A 401

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4FEY)

(-) Cis Peptide Bonds  (1, 1)

Asymmetric/Biological Unit
No.Residues
1Lys A:183 -Pro A:184

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4FEY)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4FEY)

(-) Exons   (0, 0)

(no "Exon" information available for 4FEY)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
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SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:392
                                                                                                                                                                                                                                                                                                                                                                                                                                        
               SCOP domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ....hhhhh.....eeeee......ee..ee..hhhhhhhhhhhhhhhhhh.eeeee...........hhhhhhhhhhhhhhhhhh..eeee.............eeee.hhhhh......hhhhhhhhhhh..eeee.hhhhh.......hhhhhhh.eeeehhhhhhhhhhhhhhhh.....eeeeeee.hhhhhhhhhhhhh....eeeeeehhhhhhhhhh.........hhhhhhhhhhhhhhhhhh........eeeee.........eeee.hhh....eeeeehhhhhhhhhhhhhhh.eeeee.......hhhhhhhhhhhhhhhhhhh.eeeeehhhhhhhhhhh.......eee..hhhhhhhhh...hhhhhhhhhh... Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4fey A   1 MSFLTLKDVDLKDKKVLVRVDFNVPVKDGKVTSKVRIEAAIPTIQYILDQGGAVILMSHLGRPTEGEYDSQFSLEPVAKALSEIINKPVKFAKDWLDGVDVKAGEIVMCENVRFNSGEKKSTDDLSKKIASLGDVFVMDAFATAHRAQASTYGVAKYIPVACAGILLTNEIQALEKALKSPKKPMAAIVGGSKVSTKLSVLNNLLDKVEILIVGGGIANTFIKAEGFDVGNSLYEQDLVAEATEILAKAKALGVNIPVPVDVRVAKEFSENAQAIIKKVSDVVADEMILDIGPESQKIIAELLKSANTILWNGPVGVFEFDNFAEGTKALSLAIAQSHAFSVAGGGDTIAAIEKFGIKDQVSYISTAGGAFLEFLEGKKLPAIEILKEKAIR 392
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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4FEY)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4FEY)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4FEY)

(-) Gene Ontology  (8, 8)

Asymmetric/Biological Unit(hide GO term definitions)

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  4fey
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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        PGK_FRATT | Q5NF76: 4ehj

(-) Related Entries Specified in the PDB File

4ehj APO STRUCTURE RELATED ID: IDP02760 RELATED DB: TARGETTRACK