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(-) Description

Title :  PTPN18 IN COMPLEX WITH HER2-PY1196 PHOSPHOR-PEPTIDES
 
Authors :  H. M. Wang, F. Yang, Y. J. Du, D. X. Yang, Y. Zhang, X. Yu, J. P. Sun
Date :  04 Aug 12  (Deposition) - 23 Oct 13  (Release) - 23 Oct 13  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.10
Chains :  Asym. Unit :  A,B,E,F
Biol. Unit 1:  A,F  (1x)
Biol. Unit 2:  B,E  (1x)
Keywords :  Phosphatase, Tyrosine Phosphorylation, Hydrolase, Hydrolase-Peptide Complex (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  H. M. Wang, F. Yang, Y. J. Du, D. X. Yang, Y. Zhang, X. Yu, J. P. Sun
Ptpn18-Her2 Peptides
To Be Published
PubMed: search

(-) Compounds

Molecule 1 - TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 18
    Chains: A, B
    EC Number: 3.1.3.48
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PGEX-6P2
    Expression System Strain: BL21
    Expression System Taxid: 511693
    Expression System Vector Type: PLASMID
    Fragment: UNP RESIDUES 6-300
    Gene: PTPN18, BDP1
    Mutation: YES
    Organism Common: HUMAN
    Organism Scientific: HOMO SAPIENS
    Organism Taxid: 9606
    Synonym: BRAIN-DERIVED PHOSPHATASE
 
Molecule 2 - HER2-PY1196 PHOSPHOR-PEPTIDE
    Chains: E, F
    Engineered: YES
    Organism Common: HUMAN
    Organism Scientific: HOMO SAPIENS
    Organism Taxid: 9606
    Synthetic: YES

 Structural Features

(-) Chains, Units

  1234
Asymmetric Unit : ABEF
Biological Unit 1 (1x): A  F
Biological Unit 2 (1x):  BE 

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 2)

Asymmetric Unit (1, 2)
No.NameCountTypeFull Name
1PTR2Mod. Amino AcidO-PHOSPHOTYROSINE
Biological Unit 1 (1, 1)
No.NameCountTypeFull Name
1PTR1Mod. Amino AcidO-PHOSPHOTYROSINE
Biological Unit 2 (1, 1)
No.NameCountTypeFull Name
1PTR1Mod. Amino AcidO-PHOSPHOTYROSINE

(-) Sites  (0, 0)

(no "Site" information available for 4GFV)

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4GFV)

(-) Cis Peptide Bonds  (1, 1)

Asymmetric Unit
No.Residues
1Ala B:8 -Arg B:9

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4GFV)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4GFV)

(-) Exons   (0, 0)

(no "Exon" information available for 4GFV)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:286
                                                                                                                                                                                                                                                                                                                               
               SCOP domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh....hhhhhh..hhhhh.......hhhhh.....hhhhh......eeeee.....eeeeee......hhhhhhhhhhhh...eeee....ee..ee............eee..eeeeeeeeeeee..eeeeeeeeee..eeeeeeeeee..........hhhhhhhhhhhhhhh.....eeee....hhhhhhhhhhhhhhhhhhhh......hhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                4gfv A    6 DSARSFLERLEARGGREGAVLAGEFSDIQACSAAWKADGVCSTVAGSRPENVRKNRYKDVLPYDQTRVILSLLQEEGHSDYINGNFIRGVDGSLAYIATQGPLPHTLLDFWRLVWEFGVKVILMACREIENGRKRCERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQKESRSVYQLQYMSWPDRGVPPDHMLAMVEEARRLQGSGPEPLCVHSSAGCGRTGVLCTVDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQYRFLYHTVAQMFC  293
                                    15        25        35        45        55        65        75        85        95       105       115       125       135       145       155       165       175       185       195     ||207       217       227       237       247       257       267       277       287      
                                                                                                                                                                                                                             201|                                                                                         
                                                                                                                                                                                                                              204                                                                                         


Chain B from PDB  Type:PROTEIN  Length:288
                                                                                                                                                                                                                                                                                                                                 
               SCOP domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ CATH domains
               Pfam domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Pfam domains
         Sec.struct. author ..hhhhhhhhhh..hhhhhhhhhhhhhhhhhhhhhhh....hhhhhh..hhhhh.......hhhhh.....hhhhh......eeeee.....eeeeee......hhhhhhhhhhhh...eeee....ee..ee............eee..eeeeeeeeeeee..eeeeeeeeee..eeeeeeeeee..........hhhhhhhhhhhhhhhh.....eeee....hhhhhhhhhhhhhhhhhhhh......hhhhhhhhhhh.......hhhhhhhhhhhhhhhh... Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ SAPs(SNPs)
                    PROSITE ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Transcript
                4gfv B    7 SARSFLERLEARGGREGAVLAGEFSDIQACSAAWKADGVCSTVAGSRPENVRKNRYKDVLPYDQTRVILSLLQEEGHSDYINGNFIRGVDGSLAYIATQGPLPHTLLDFWRLVWEFGVKVILMACREIENGRKRCERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQKESRSVYQLQYMSWPDRGVPSPDHMLAMVEEARRLQGSGPEPLCVHSSAGCGRTGVLCTVDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQYRFLYHTVAQMFCST  295
                                    16        26        36        46        56        66        76        86        96       106       116       126       136       146       156       166       176       186       196    || 207       217       227       237       247       257       267       277       287        
                                                                                                                                                                                                                            201|                                                                                            
                                                                                                                                                                                                                             203                                                                                            


Chain E from PDB  Type:PROTEIN  Length:6
                                       
               SCOP domains ------ SCOP domains
               CATH domains ------ CATH domains
               Pfam domains ------ Pfam domains
         Sec.struct. author ...... Sec.struct. author
                 SAPs(SNPs) ------ SAPs(SNPs)
                    PROSITE ------ PROSITE
                 Transcript ------ Transcript
                4gfv E 1194 PEyLTP 1199
                              |   
                           1196-PTR


Chain F from PDB  Type:PROTEIN  Length:5
                                      
               SCOP domains ----- SCOP domains
               CATH domains ----- CATH domains
               Pfam domains ----- Pfam domains
         Sec.struct. author ..... Sec.struct. author
                 SAPs(SNPs) ----- SAPs(SNPs)
                    PROSITE ----- PROSITE
                 Transcript ----- Transcript
                4gfv F 1194 PEyLT 1198
                              |  
                              |  
                           1196-PTR
   Legend:   → Mismatch (orange background)
  - → Gap (green background, '-', border residues have a numbering label)
    → Modified Residue (blue background, lower-case, 'x' indicates undefined single-letter code, labelled with number + name)
  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4GFV)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4GFV)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4GFV)

(-) Gene Ontology  (11, 11)

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    Ala B:8 - Arg B:9   [ RasMol ]  
 
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 Related Entries

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        PTN18_HUMAN | Q99952: 2oc3 4gfu 4nnd

(-) Related Entries Specified in the PDB File

4gfu 4gfw