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(-) Description

Title :  CRYSTAL STRUCTURE OF APO HUMAN PRPF4B KINASE DOMAIN
 
Authors :  I. Mechin, K. Haas, X. Chen, Y. Zhang, L. Mclean
Date :  06 Dec 12  (Deposition) - 28 Aug 13  (Release) - 06 Nov 13  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.44
Chains :  Asym./Biol. Unit :  A,B
Keywords :  Kinase, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  Q. Gao, I. Mechin, N. Kothari, Z. Guo, G. Deng, K. Haas, J. Mcmanus, D. Hoffmann, A. Wang, D. Wiederschain, J. Rocnik, W. Czechtizky, X. Chen, L. Mclean, H. Arlt, D. Harper, F. Liu, T. Majid, V. Patel, C. Lengauer, C. Garcia-Echeverria, B. Zhang, H. Cheng, M. Dorsch, S. M. Huang
Evaluation Of Cancer Dependence And Druggability Of Prp4 Kinase Using Cellular, Biochemical, And Structural Approaches.
J. Biol. Chem. V. 288 30125 2013
PubMed-ID: 24003220  |  Reference-DOI: 10.1074/JBC.M113.473348

(-) Compounds

Molecule 1 - SERINE/THREONINE-PROTEIN KINASE PRP4 HOMOLOG
    Chains: A, B
    EC Number: 2.7.11.1
    Engineered: YES
    Expression System: SPODOPTERA FRUGIPERDA
    Expression System Plasmid: PFASTBAC1
    Expression System Strain: SF9
    Expression System Taxid: 7108
    Expression System Vector Type: PLASMID
    Fragment: KINASE DOMAIN
    Gene: KIAA0536, PRP4, PRP4H, PRP4K, PRPF4B
    Organism Common: HUMAN
    Organism Scientific: HOMO SAPIENS
    Organism Taxid: 9606
    Synonym: PRP4 KINASE, PRP4 PRE-MRNA-PROCESSING FACTOR 4 HOMOLOG

 Structural Features

(-) Chains, Units

  12
Asymmetric/Biological Unit : AB

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (2, 8)

Asymmetric/Biological Unit (2, 8)
No.NameCountTypeFull Name
1PTR2Mod. Amino AcidO-PHOSPHOTYROSINE
2SO46Ligand/IonSULFATE ION

(-) Sites  (6, 6)

Asymmetric Unit (6, 6)
No.NameEvidenceResiduesDescription
1AC1SOFTWARESER A:993 , ASN A:995 , GLN A:996 , SO4 A:1105BINDING SITE FOR RESIDUE SO4 A 1101
2AC2SOFTWARELYS A:747 , PHE A:748 , ARG A:808BINDING SITE FOR RESIDUE SO4 A 1102
3AC3SOFTWAREPTR A:849 , ARG A:853 , ARG A:856 , HOH A:1217BINDING SITE FOR RESIDUE SO4 A 1103
4AC4SOFTWAREARG A:755BINDING SITE FOR RESIDUE SO4 A 1104
5AC5SOFTWAREARG A:991 , ILE A:992 , SER A:993 , GLN A:996 , SO4 A:1101 , SER B:993 , ASN B:995 , GLN B:996BINDING SITE FOR RESIDUE SO4 A 1105
6AC6SOFTWAREPTR B:849 , ARG B:853 , ARG B:856BINDING SITE FOR RESIDUE SO4 B 1101

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4IAN)

(-) Cis Peptide Bonds  (1, 1)

Asymmetric/Biological Unit
No.Residues
1Gly B:696 -Val B:697

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4IAN)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4IAN)

(-) Exons   (0, 0)

(no "Exon" information available for 4IAN)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:322
                                                                                                                                                                                                                                                                                                                                                                   
               SCOP domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ......ee...eeeeeeee....eeeeeeee.hhh..eeeeeee....hhhhhhhhhhhhhhhhhhh..........eeeeeee..eeeeee....eehhhhhhh.......hhhhhhhhhhhhhhhhhhhhhh.ee....hhh.eee......eee......ee...........hhhhhhhhhhhh....hhhhhhhhhhhhhhhhhh.......hhhhhhhhhhhhhh..hhhhhhh..hhhhh......eeeee....eeeee...............hhhhhhhhhhhhhhhhhhh..hhhhh.hhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                4ian A  676 RVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPyLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDREKVTVMSTINPTKDLLADLILPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQE 1005
                                   685       695       705       715       725       735       745       755       765       775       785       795       805       815       825       835       845   |   855       865       875       885       895       905       915       925       935|      949       959||     973       983       993      1003  
                                                                                                                                                                                                       849-PTR                                                                               935|                 960|                                        
                                                                                                                                                                                                                                                                                              940                  965                                        


Chain B from PDB  Type:PROTEIN  Length:320
                                                                                                                                                                                                                                                                                                                                                                 
               SCOP domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ......ee...eeeeeeeee...eeeeeeee.hhh..eeeeeee....hhhhhhhhhhhhhhhhhhhh.........eeeeeee..eeeeee....eehhhhhhhh......hhhhhhhhhhhhhhhhhhhhhh.ee....hhh.eee......eee......ee...........hhhhhhhhhhhh....hhhhhhhhhhhhhhhhhh.......hhhhhhhhhhhhhh..hhhhhhhh.hhhhhh.....eeeee...eeeee.......hhhhhh....hhhhhhhhhhhhhhhh..hhhhh.hhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                4ian B  676 RVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPyLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVREKVTVMSTINPTKDLLADLIGCDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQE 1005
                                   685       695       705       715       725       735       745       755       765       775       785       795       805       815       825       835       845   |   855       865       875       885       895       905       915       925       940       950       960 ||    975       985       995      1005
                                                                                                                                                                                                       849-PTR                                                                              934|                   962|                                     
                                                                                                                                                                                                                                                                                             940                    968
   Legend:   → Mismatch (orange background)
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  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4IAN)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4IAN)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4IAN)

(-) Gene Ontology  (17, 17)

Asymmetric/Biological Unit(hide GO term definitions)

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  Cis Peptide Bonds
    Gly B:696 - Val B:697   [ RasMol ]  
 

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  4ian
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  PRP4B_HUMAN | Q13523
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  2.7.11.1
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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        PRP4B_HUMAN | Q13523: 4ifc 4iir 4ijp

(-) Related Entries Specified in the PDB File

4ifc 4iir 4ijp