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(-) Description

Title :  CRYSTAL STRUCTURE OF AN H329A MUTANT OF P. AERUGINOSA PMM/PGM
 
Authors :  Y. Lee, R. Mehra-Chaudhary, C. Furdui, L. Beamer
Date :  29 Dec 12  (Deposition) - 14 Aug 13  (Release) - 14 Aug 13  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.80
Chains :  Asym./Biol. Unit :  A
Keywords :  A-D-Phosphohexomutase, Isomerase, Phosphoserine (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  Y. Lee, R. Mehra-Chaudhary, C. Furdui, L. J. Beamer
Identification Of An Essential Active-Site Residue In The Alpha-D-Phosphohexomutase Enzyme Superfamily.
Febs J. V. 280 2622 2013
PubMed-ID: 23517223  |  Reference-DOI: 10.1111/FEBS.12249

(-) Compounds

Molecule 1 - PHOSPHOMANNOMUTASE/PHOSPHOGLUCOMUTASE
    Chains: A
    EC Number: 5.4.2.2, 5.4.2.8
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Strain: BL21(DE3)
    Expression System Taxid: 562
    Expression System Vector Type: PET3A
    Gene: ALGC, PA5322
    Mutation: YES
    Organism Scientific: PSEUDOMONAS AERUGINOSA
    Organism Taxid: 208964
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228
    Synonym: PMM / PGM

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit : A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (3, 4)

Asymmetric/Biological Unit (3, 4)
No.NameCountTypeFull Name
1GOL2Ligand/IonGLYCEROL
2MG1Ligand/IonMAGNESIUM ION
3SEP1Mod. Amino AcidPHOSPHOSERINE

(-) Sites  (3, 3)

Asymmetric Unit (3, 3)
No.NameEvidenceResiduesDescription
1AC1SOFTWARESEP A:108 , ASP A:242 , ASP A:244 , ASP A:246 , HOH A:628BINDING SITE FOR RESIDUE MG A 501
2AC2SOFTWAREASN A:95 , PHE A:337 , HOH A:620 , HOH A:648 , HOH A:772 , HOH A:816 , HOH A:875BINDING SITE FOR RESIDUE GOL A 502
3AC3SOFTWAREARG A:135 , GLU A:139 , TYR A:413 , GLU A:440 , HOH A:623BINDING SITE FOR RESIDUE GOL A 503

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4IL8)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4IL8)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4IL8)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4IL8)

(-) Exons   (0, 0)

(no "Exon" information available for 4IL8)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:459
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
               SCOP domains d4il8a1 A:5-154 automated matches                                                                                                                     d4il8a2 A:155-258 automated matches                                                                     d4il8a3 A:259-367 automated matches                                                                          d4il8a4 A:368-463 automated matches                                                              SCOP domains
               CATH domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .....hhhhh....eeee.....hhhhhhhhhhhhhhhhhhh...eeeeee....hhhhhhhhhhhhhhh...eeeeeee.hhhhhhhhhhhh...eeeee........eeeeeeee..ee..hhhhhhhhhhhhh........eeee..hhhhhhhhhhhhh.....eeeeee...hhhhhhhhhhhhhhheeeeee................hhhhhhhhhhhhhhh...eeeee......eeeee....eehhhhhhhhhhhhhhhhh...eeeee....hhhhhhhhhh..eeeee..hhhhhhhhhhhhh..eee.....eee........hhhhhhhhhhhhhhh...hhhhhhhh....ee...eeee....hhhhhhhhhhhhh.....eee....eeeee..eeeeeee.....eeeeeeee.hhhhhhhhhhhhhhhhhhhh....... Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4il8 A   5 KAPTLPASIFRAYDIRGVVGDTLTAETAYWIGRAIGSESLARGEPCVAVGRDGRLSGPELVKQLIQGLVDCGCQVSDVGMVPTPVLYYAANVLEGKSGVMLTGsHNPPDYNGFKIVVAGETLANEQIQALRERIEKNDLASGVGSVEQVDILPRYFKQIRDDIAMAKPMKVVVDCGNGVAGVIAPQLIEALGCSVIPLYCEVDGNFPNHHPDPGKPENLKDLIAKVKAENADLGLAFDGDGDRVGVVTNTGTIIYPDRLLMLFAKDVVSRNPGADIIFDVKCTRRLIALISGYGGRPVMWKTGHSLIKKKMKETGALLAGEMSGAVFFKERWFGFDDGIYSAARLLEILSQDQRDSEHVFSAFPSDISTPEINITVTEDSKFAIIEALQRDAQWGEGNITTLDGVRVDYPKGWGLVRASNTTPVLVLRFEADTEEELERIKTVFRNQLKAVDSSLPVPF 463
                                    14        24        34        44        54        64        74        84        94       104   |   114       124       134       144       154       164       174       184       194       204       214       224       234       244       254       264       274       284       294       304       314       324       334       344       354       364       374       384       394       404       414       424       434       444       454         
                                                                                                                                 108-SEP
   Legend:   → Mismatch (orange background)
  - → Gap (green background, '-', border residues have a numbering label)
    → Modified Residue (blue background, lower-case, 'x' indicates undefined single-letter code, labelled with number + name)
  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (2, 4)

Asymmetric/Biological Unit
(-)
Class: Alpha and beta proteins (a+b) (23004)
(-)
Class: Alpha and beta proteins (a/b) (23833)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4IL8)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4IL8)

(-) Gene Ontology  (17, 17)

Asymmetric/Biological Unit(hide GO term definitions)

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        ALGC_PSEAE | P26276: 1k2y 1k35 1p5d 1p5g 1pcj 1pcm 2fkf 2fkm 2h4l 2h5a 3bkq 3c04 3rsm 4mrq

(-) Related Entries Specified in the PDB File

1k2y1k351p5d1p5g1pcj
1pcm