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(-) Description

Title :  STRUCTURE OF DUSP8
 
Authors :  D. G. Jeong, S. J. Kim, S. E. Ryu
Date :  14 Mar 13  (Deposition) - 26 Feb 14  (Release) - 26 Feb 14  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.90
Chains :  Asym. Unit :  A,B
Biol. Unit 1:  A  (1x)
Biol. Unit 2:  B  (1x)
Keywords :  Alpha/Beta Hydrolase, Hydrolase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  D. G. Jeong, C. H. Wei, B. Ku, T. J. Jeon, P. N. Chien, J. K. Kim, S. Y. Park, H. S. Hwang, S. Y. Ryu, H. Park, D. S. Kim, S. J. Kim, S. E. Ryu
The Family-Wide Structure And Function Of Human Dual-Specificity Protein Phosphatases
Acta Crystallogr. , Sect. D V. 70 421 2014
PubMed: search  |  Reference-DOI: 10.1107/S1399004713029866

(-) Compounds

Molecule 1 - DUAL SPECIFICITY PROTEIN PHOSPHATASE 8
    Chains: A, B
    EC Number: 3.1.3.16, 3.1.3.48
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Taxid: 562
    Expression System Vector Type: PLASMID
    Fragment: UNP RESIDUES 160-310
    Gene: DUSP8, C11ORF81, VH5
    Mutation: YES
    Organism Common: HUMAN
    Organism Scientific: HOMO SAPIENS
    Organism Taxid: 9606
    Synonym: DUAL SPECIFICITY PROTEIN PHOSPHATASE HVH-5

 Structural Features

(-) Chains, Units

  12
Asymmetric Unit : AB
Biological Unit 1 (1x): A 
Biological Unit 2 (1x):  B

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 4)

Asymmetric Unit (1, 4)
No.NameCountTypeFull Name
1SO44Ligand/IonSULFATE ION
Biological Unit 1 (1, 2)
No.NameCountTypeFull Name
1SO42Ligand/IonSULFATE ION
Biological Unit 2 (1, 2)
No.NameCountTypeFull Name
1SO42Ligand/IonSULFATE ION

(-) Sites  (4, 4)

Asymmetric Unit (4, 4)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREASP A:215 , SER A:246 , LEU A:247 , ALA A:248 , GLY A:249 , ILE A:250 , SER A:251 , ARG A:252BINDING SITE FOR RESIDUE SO4 A 501
2AC2SOFTWAREASN A:195 , SER A:196 , ASN A:214 , ARG A:252 , HOH A:607BINDING SITE FOR RESIDUE SO4 A 502
3AC3SOFTWAREASP B:215 , SER B:246 , LEU B:247 , ALA B:248 , GLY B:249 , ILE B:250 , SER B:251 , ARG B:252 , HOH B:501BINDING SITE FOR RESIDUE SO4 B 401
4AC4SOFTWARESER B:194 , ASN B:195 , SER B:196 , ASN B:214 , ARG B:252 , HOH B:541BINDING SITE FOR RESIDUE SO4 B 402

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4JMK)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4JMK)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4JMK)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4JMK)

(-) Exons   (0, 0)

(no "Exon" information available for 4JMK)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:151
                                                                                                                                                                                       
               SCOP domains d4jmka_ A: automated matches                                                                                                                            SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ..eeee..eeeehhhhhhhhhhhhhh.eeeeee...........hhh.eee..........hhhhhhhhhhhhhhhhhh..eeeee.....hhhhhhhhhhhhhhhh.hhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhhh... Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4jmk A 160 GLTRILPHLYLGSQKDVLNKDLMTQNGISYVLNASNSCPKPDFICESRFMRVPINDNYCEKLLPWLDKSIEFIDKAKLSSCQVIVHSLAGISRSATIAIAYIMKTMGMSSDDAYRFVKDRRPSISPNFNFLGQLLEYERSLKLLAALQGDP 310
                                   169       179       189       199       209       219       229       239       249       259       269       279       289       299       309 


Chain B from PDB  Type:PROTEIN  Length:145
                                                                                                                                                                                 
               SCOP domains d4jmkb_ B: automated matches                                                                                                                      SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ...eeee..eeeehhhhhhhhhhhhhh.eeeeee...........hhh.eee..........hhhhhhhhhhhhhhhhhh..eeeee.....hhhhhhhhhhhhhhhh.hhhhhhhhhhhhh.....hhhhhhhhhhhhhhhh.. Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4jmk B 159 MGLTRILPHLYLGSQKDVLNKDLMTQNGISYVLNASNSCPKPDFICESRFMRVPINDNYCEKLLPWLDKSIEFIDKAKLSSCQVIVHSLAGISRSATIAIAYIMKTMGMSSDDAYRFVKDRRPSISPNFNFLGQLLEYERSLKLL 303
                                   168       178       188       198       208       218       228       238       248       258       268       278       288       298
   Legend:   → Mismatch (orange background)
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  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (1, 2)

Asymmetric Unit
(-)
Class: Alpha and beta proteins (a/b) (23833)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4JMK)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4JMK)

(-) Gene Ontology  (12, 12)

Asymmetric Unit(hide GO term definitions)

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 Related Entries

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(-) Related Entries Specified in the PDB File

4jmj 4ki9