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(-) Description

Title :  HIGH-RESOLUTION STRUCTURE OF C DOMAIN OF STAPHYLOCOCCAL PROTEIN A AT ROOM TEMPERATURE
 
Authors :  L. N. Deis, C. W. Pemble Iv, T. G. Oas, J. S. Richardson, D. C. Richardson
Date :  21 Nov 13  (Deposition) - 08 Oct 14  (Release) - 22 Oct 14  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.42
Chains :  Asym./Biol. Unit :  A
Keywords :  Spa, Three-Helix Bundle, Conformational Heterogeneity, Rapidly Unfolding And Folding, Ruf, Antibody Binding, Antibody, Protein Binding (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  L. N. Deis, C. W. Pemble, Y. Qi, A. Hagarman, D. C. Richardson, J. S. Richardson, T. G. Oas
Multiscale Conformational Heterogeneity In Staphylococcal Protein A: Possible Determinant Of Functional Plasticity.
Structure V. 22 1467 2014
PubMed-ID: 25295398  |  Reference-DOI: 10.1016/J.STR.2014.08.014

(-) Compounds

Molecule 1 - IMMUNOGLOBULIN G-BINDING PROTEIN A
    Chains: A
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PAED4
    Expression System Strain: BL21(DE3)
    Expression System Taxid: 469008
    Expression System Vector Type: PLASMID
    Fragment: UNP RESIDUES 270-327
    Gene: PROTEIN A, SPA
    Organism Scientific: STAPHYLOCOCCUS AUREUS
    Organism Taxid: 1280
    Other Details: C DOMAIN OF STAPHYLOCOCCAL PROTEIN A
    Strain: 8325-4
    Synonym: IGG-BINDING PROTEIN A, STAPHYLOCOCCAL PROTEIN A

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit : A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (2, 2)

Asymmetric/Biological Unit (2, 2)
No.NameCountTypeFull Name
1SCN1Ligand/IonTHIOCYANATE ION
2ZN1Ligand/IonZINC ION

(-) Sites  (2, 2)

Asymmetric Unit (2, 2)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREGLU A:47 , LYS A:58 , SCN A:102BINDING SITE FOR RESIDUE ZN A 101
2AC2SOFTWAREGLY A:29 , SER A:33 , GLU A:47 , LYS A:58 , ZN A:101BINDING SITE FOR RESIDUE SCN A 102

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4NPE)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4NPE)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4NPE)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4NPE)

(-) Exons   (0, 0)

(no "Exon" information available for 4NPE)

(-) Sequences/Alignments

Asymmetric/Biological Unit
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SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:58
                                                                                         
               SCOP domains ---------------------------------------------------------- SCOP domains
               CATH domains ---------------------------------------------------------- CATH domains
               Pfam domains ---------------------------------------------------------- Pfam domains
         Sec.struct. author .....hhhhhhhhhhhhhh...hhhhhhhhhhhhhhh..hhhhhhhhhhhhhhhh... Sec.struct. author
                 SAPs(SNPs) ---------------------------------------------------------- SAPs(SNPs)
                    PROSITE ---------------------------------------------------------- PROSITE
                 Transcript ---------------------------------------------------------- Transcript
                  4npe A  1 ADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPK 58
                                    10        20        30        40        50
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 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4NPE)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4NPE)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4NPE)

(-) Gene Ontology  (6, 6)

Asymmetric/Biological Unit(hide GO term definitions)

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        SPA_STAAU | P38507: 1bdc 1bdd 1edi 1edj 1edk 1edl 1fc2 1h0t 1lp1 1q2n 1ss1 2jwd 2m5a 2spz 3mzw 4npd 4npf 4wwi 4y4y 4y5z 4zmd 4znc 5cbn 5cbo 5coc 5ewx 5h75 5h76 5h77 5h79 5h7a 5h7b 5h7c 5h7d 5x3f 5xby

(-) Related Entries Specified in the PDB File

4npd 4npf