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(-) Description

Title :  SAICAR SYNTHETASE (TYPE-2) IN COMPLEX WITH ATP AND TDP
 
Authors :  K. Manjunath, J. Jeyakanthan, K. Sekar
Date :  26 Dec 13  (Deposition) - 31 Dec 14  (Release) - 31 Dec 14  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.20
Chains :  Asym. Unit :  A
Biol. Unit 1:  A  (2x)
Keywords :  Saicar Synthetase-Like Fold, Ligase, Atp Binding, Cair Binding, Aspartate Binding (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  K. Manjunath, J. Jeyakanthan, K. Sekar
Saicar Synthetase (Type-2) In Complex With Atp And Tdp
To Be Published
PubMed: search

(-) Compounds

Molecule 1 - PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE
    Chains: A
    EC Number: 6.3.2.6
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PET11A
    Expression System Strain: BL21-CODONPLUS (DE3)-RIL
    Expression System Taxid: 562
    Expression System Vector Type: PLASMID
    Gene: PH0239, PURC
    Organism Scientific: PYROCOCCUS HORIKOSHII
    Organism Taxid: 70601
    Strain: OT3
    Synonym: SAICAR SYNTHETASE

 Structural Features

(-) Chains, Units

  1
Asymmetric Unit : A
Biological Unit 1 (2x): A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (4, 4)

Asymmetric Unit (4, 4)
No.NameCountTypeFull Name
1ACT1Ligand/IonACETATE ION
2ATP1Ligand/IonADENOSINE-5'-TRIPHOSPHATE
3PO41Ligand/IonPHOSPHATE ION
4TYD1Ligand/IonTHYMIDINE-5'-DIPHOSPHATE
Biological Unit 1 (4, 8)
No.NameCountTypeFull Name
1ACT2Ligand/IonACETATE ION
2ATP2Ligand/IonADENOSINE-5'-TRIPHOSPHATE
3PO42Ligand/IonPHOSPHATE ION
4TYD2Ligand/IonTHYMIDINE-5'-DIPHOSPHATE

(-) Sites  (4, 4)

Asymmetric Unit (4, 4)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREGLU A:89 , ARG A:93 , GLY A:98 , SER A:99 , ASP A:173 , PHE A:174 , LYS A:175 , ASP A:195 , THR A:196 , CYS A:197 , ARG A:198 , ARG A:214 , PO4 A:303 , HOH A:422 , HOH A:483 , HOH A:489BINDING SITE FOR RESIDUE TYD A 301
2AC2SOFTWAREGLY A:10 , LYS A:11 , ALA A:12 , LYS A:13 , MET A:15 , HIS A:68 , GLU A:81 , LEU A:83 , MET A:85 , LYS A:122 , GLU A:177 , ASP A:190BINDING SITE FOR RESIDUE ATP A 302
3AC3SOFTWARETHR A:32 , ALA A:33 , PHE A:34 , LYS A:210 , ARG A:214 , TYD A:301BINDING SITE FOR RESIDUE PO4 A 303
4AC4SOFTWAREARG A:103 , PRO A:129 , MET A:130BINDING SITE FOR RESIDUE ACT A 304

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4O7W)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4O7W)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4O7W)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4O7W)

(-) Exons   (0, 0)

(no "Exon" information available for 4O7W)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:232
                                                                                                                                                                                                                                                                        
               SCOP domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .ee...eeeee....eeeeee..eeee....eeee..hhhhhhhhhhhhhhhhhhhh.....eeee....eeeee..ee..eeeeeeee.hhhhhhh.......eeeeeeeeeee........eehhhhhhhh..hhhhhhhhhhhhhhhhhhhhhhhhh..eeeeeeee.eee.....eee........eeeee........hhhhhh...hhhhhhhhhhhhhhh..... Sec.struct. author
                 SAPs(SNPs) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4o7w A   7 VYEGKAKKMIPIDDDKLIMEFKDDATAFDGTKKARFKGKGWLNAQLSVIFFKLLEEHGIKTHFIGVAGGNRLIVEKLDMYPLEVVVRNVVAGSLKKRLPLPEGYELPEPIVELYYKNDELHDPMINYYHAKVLGISLDEIKKIEEIALKVNEILKDYLAKKGIILVDFKLEFGKDKNGDIVLADEISPDTCRFWDAKTKRSLDKDVFRFDKGDLIEAYKEIYERITGEKPEF 238
                                    16        26        36        46        56        66        76        86        96       106       116       126       136       146       156       166       176       186       196       206       216       226       236
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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4O7W)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4O7W)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4O7W)

(-) Gene Ontology  (6, 6)

Asymmetric Unit(hide GO term definitions)

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  4o7w
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  PUR7_PYRHO | O57978
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  6.3.2.6
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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        PUR7_PYRHO | O57978: 3u54 3u55 4o7l 4o7n 4o7r 4o7s 4o7t 4o7v 4o7y 4o7z 4o81 4o82 4o83 4o84 4o86

(-) Related Entries Specified in the PDB File

3u54 3u55 4o7l 4o7n 4o7r 4o7s 4o7t 4o7v 4o7y 4o7z 4o81 4o82 4o83 4o84 4o86