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(-) Description

Title :  INFLUENZA A M2 WILD TYPE TM DOMAIN AT LOW PH IN THE LIPIDIC CUBIC PHASE UNDER CRYO DIFFRACTION CONDITIONS
 
Authors :  J. L. Thomaston, W. F. Degrado
Date :  05 Jun 14  (Deposition) - 11 Nov 15  (Release) - 16 Dec 15  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.10
Chains :  Asym. Unit :  A
Biol. Unit 1:  A  (4x)
Keywords :  Transmembrane Alpha Helix, Ph-Activated Proton Channel, Viral Protein (Keyword Search: [Gene Ontology, PubMed, Web (Google))
 
Reference :  J. L. Thomaston, M. Alfonso-Prieto, R. A. Woldeyes, J. S. Fraser, M. L. Klein, G. Fiorin, W. F. Degrado
High-Resolution Structures Of The M2 Channel From Influenza A Virus Reveal Dynamic Pathways For Proton Stabilization An Transduction.
Proc. Natl. Acad. Sci. Usa V. 112 14260 2015
PubMed-ID: 26578770  |  Reference-DOI: 10.1073/PNAS.1518493112

(-) Compounds

Molecule 1 - INFLUENZA M2 MONOMER
    ChainsA
    EngineeredYES
    Organism ScientificINFLUENZA A VIRUS
    Organism Taxid385599
    Other DetailsTHIS SEQUENCE IS FOUND IN THE INFLUENZA A VIRUS (STRAIN A/UDORN/307/1972 H3N2) AND WAS MANUALLY SYNTHESIZED USING FMOC CHEMISTRY. N- AND C-TERMINAL MODIFICATIONS ARE PRESENT.
    SyntheticYES

 Structural Features

(-) Chains, Units

  1
Asymmetric Unit A
Biological Unit 1 (4x)A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (6, 8)

Asymmetric Unit (6, 8)
No.NameCountTypeFull Name
1ACE1Mod. Amino AcidACETYL GROUP
2CA2Ligand/IonCALCIUM ION
3CL1Ligand/IonCHLORIDE ION
4EDO1Ligand/Ion1,2-ETHANEDIOL
5NH21Mod. Amino AcidAMINO GROUP
6OLB2Ligand/Ion(2S)-2,3-DIHYDROXYPROPYL (9Z)-OCTADEC-9-ENOATE
Biological Unit 1 (4, 20)
No.NameCountTypeFull Name
1ACE4Mod. Amino AcidACETYL GROUP
2CA-1Ligand/IonCALCIUM ION
3CL-1Ligand/IonCHLORIDE ION
4EDO4Ligand/Ion1,2-ETHANEDIOL
5NH24Mod. Amino AcidAMINO GROUP
6OLB8Ligand/Ion(2S)-2,3-DIHYDROXYPROPYL (9Z)-OCTADEC-9-ENOATE

(-) Sites  (6, 6)

Asymmetric Unit (6, 6)
No.NameEvidenceResiduesDescription
1AC1SOFTWARESER A:22 , HOH A:201 , HOH A:242BINDING SITE FOR RESIDUE CA A 101
2AC2SOFTWARESER A:23BINDING SITE FOR RESIDUE CL A 102
3AC3SOFTWAREASP A:24 , OLB A:105 , HOH A:207 , HOH A:211 , HOH A:212 , HOH A:220BINDING SITE FOR RESIDUE CA A 103
4AC4SOFTWAREHIS A:37 , TRP A:41 , HOH A:223 , HOH A:224 , HOH A:227BINDING SITE FOR RESIDUE EDO A 104
5AC5SOFTWAREASP A:24 , PRO A:25 , LEU A:26 , ILE A:35 , LEU A:38 , ILE A:39 , ILE A:42 , LEU A:43 , ARG A:45 , NH2 A:47 , CA A:103 , OLB A:106 , HOH A:207 , HOH A:211 , HOH A:220BINDING SITE FOR RESIDUE OLB A 105
6AC6SOFTWAREPRO A:25 , VAL A:28 , ALA A:29 , ILE A:33 , HIS A:37 , ILE A:39 , LEU A:40 , ARG A:45 , OLB A:105 , HOH A:206 , HOH A:240BINDING SITE FOR RESIDUE OLB A 106

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4QKC)

(-) Cis Peptide Bonds  (0, 0)

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 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

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(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4QKC)

(-) Exons   (0, 0)

(no "Exon" information available for 4QKC)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
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Chain A from PDB  Type:PROTEIN  Length:27
                                                          
               SCOP domains --------------------------- SCOP domains
               CATH domains --------------------------- CATH domains
               Pfam domains --------------------------- Pfam domains
         Sec.struct. author ...hhhhhhhhhhhhhhhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) --------------------------- SAPs(SNPs)
                    PROSITE --------------------------- PROSITE
                 Transcript --------------------------- Transcript
                  4qkc A 21 xSSDPLVVAASIIGILHLILWILDRLx 47
                            |       30        40      |
                            |                        47-NH2
                           21-ACE                      

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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4QKC)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4QKC)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4QKC)

(-) Gene Ontology  (18, 31)

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        M2_I72A2 | P0DOF51mp6 2kih 2kwx 2l0j 2n70 2rlf 4qk7 4qkl 4qkm 5c02
        W8PGZ1_9INFA | W8PGZ14qk7 4qkl 4qkm

(-) Related Entries Specified in the PDB File

4qk6 4qk7 4qkl 4qkm