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(-) Description

Title :  CRYSTAL STRUCTURE OF IMPORTIN-ALPHA FROM NEUROSPORA CRASSA COMPLEXED WITH SV40NLS
 
Authors :  N. E. Bernardes, A. A. S. Takeda, M. R. M. Fontes
Date :  11 Dec 14  (Deposition) - 01 Jul 15  (Release) - 01 Jul 15  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.76
Chains :  Asym./Biol. Unit :  A,B,C
Keywords :  Arm Repeat, Nls, Nuclear Import, Importin-Alpha, Transport Protein (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  N. E. Bernardes, A. A. Takeda, T. R. Dreyer, F. Z. Freitas, M. C. Bertolini, M. R. Fontes
Structure Of Importin-Alpha From A Filamentous Fungus In Complex With A Classical Nuclear Localization Signal.
Plos One V. 10 28687 2015
PubMed-ID: 26091498  |  Reference-DOI: 10.1371/JOURNAL.PONE.0128687

(-) Compounds

Molecule 1 - IMPORTIN SUBUNIT ALPHA
    Chains: B
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PET28A
    Expression System Strain: ROSETTA(DE3) PLYSS
    Expression System Taxid: 562
    Gene: 3H10.030
    Organism Scientific: NEUROSPORA CRASSA
    Organism Taxid: 5141
    Strain: FGSC 9718
 
Molecule 2 - LARGE T ANTIGEN
    Chains: A, C
    EC Number: 3.6.4.-
    Engineered: YES
    Organism Common: SV40
    Organism Scientific: SIMIAN VIRUS 40
    Organism Taxid: 10633
    Other Details: LARGE T-ANTIGEN NUCLEAR LOCALIZATION SEQUENCE
    Synonym: LT, LT-AG
    Synthetic: YES

 Structural Features

(-) Chains, Units

  123
Asymmetric/Biological Unit : ABC

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (0, 0)

(no "Ligand,Modified Residues,Ions" information available for 4RXH)

(-) Sites  (0, 0)

(no "Site" information available for 4RXH)

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4RXH)

(-) Cis Peptide Bonds  (1, 1)

Asymmetric/Biological Unit
No.Residues
1Thr B:241 -Pro B:242

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4RXH)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4RXH)

(-) Exons   (0, 0)

(no "Exon" information available for 4RXH)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:5
                                     
               SCOP domains ----- SCOP domains
               CATH domains ----- CATH domains
               Pfam domains ----- Pfam domains
         Sec.struct. author ..... Sec.struct. author
                 SAPs(SNPs) ----- SAPs(SNPs)
                    PROSITE ----- PROSITE
                 Transcript ----- Transcript
                 4rxh A 128 KKRKV 132


Chain B from PDB  Type:PROTEIN  Length:421
                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
               SCOP domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author hhhhhhhhhh..hhhhhhhhhhhhhhhhh.....hhhhhhhh.hhhhhhhhh...hhhhhhhhhhhhhhhh..hhhhhhhhhhhhhhhhhhhhh...hhhhhhhhhhhhhhhhh.hhhhhhhhhhh.hhhhhhhhhhhh.hhhhhhhhhhhhhhhhh......hhhhhhhhhhhhhhhh...hhhhhhhhhhhhhhhh..hhhhhhhhhhh.hhhhhhhhh...hhhhhhhhhhhhhhhh..hhhhhhhhhhhhhhhhhhhhh...hhhhhhhhhhhhhhhhh.hhhhhhhhhhh.hhhhhhhhhhh.hhhhhhhhhhhhhhh.......hhhhhhhhhh.hhhhhhhhhhh.hhhhhhhhhhhhhhhhhhhhhhhhh...hhhhhhhhhhhhhhhhhhhhhh....hhhhhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4rxh B  82 DLPKMVEGVFSSEIDKQIQATTKFRKLLSKERNPPIEEVIKTGVVGRFVEFLRSPHTLVQFEAAWALTNIASGSATQTQVVIEAGAVPIFVELLGSPEPDVREQAVWALGNIAGDSPQCRDYVLSCGALRPLLTLLGDSRKLSMLRNATWTLSNFCRGKTPQPDWNTIAPALPVLAKLVYSLDDEVLIDACWAISYLSDGSNDKIQAVIEAGIPRRLVELLMHASTSVQTPALRSVGNIVTGDDVQTQVIINCGALPCLLSLLSSNKDGIRKEACWTISNITAGNSAQIQSVIDANIIPPLIHLLSHADLKTRKEACWAISNATSGGLQKPDQIRYLVAQGCIKPLCDLLACPDNKIIQVALDGLENILKVGELDKNAASINRYALFIEECGGMEKIHDCQTNANEEIYMKAYNIIEKYFS 507
                                    91       101       111       121       131       141       151       161       171       181       191       201       211       221       231       241       251       261       271       281       291       301       311       321       331       341       351       361       371       381       391       401       411       421       431       441       451       466       476       486       496       506 
                                                                                                                                                                                                                                                                                                                                                                                                                    460|                                         
                                                                                                                                                                                                                                                                                                                                                                                                                     466                                         


Chain C from PDB  Type:PROTEIN  Length:8
                                        
               SCOP domains -------- SCOP domains
               CATH domains -------- CATH domains
               Pfam domains -------- Pfam domains
         Sec.struct. author ........ Sec.struct. author
                 SAPs(SNPs) -------- SAPs(SNPs)
                    PROSITE -------- PROSITE
                 Transcript -------- Transcript
                 4rxh C 125 PPKKKRKV 132
   Legend:   → Mismatch (orange background)
  - → Gap (green background, '-', border residues have a numbering label)
    → Modified Residue (blue background, lower-case, 'x' indicates undefined single-letter code, labelled with number + name)
  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4RXH)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4RXH)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4RXH)

(-) Gene Ontology  (32, 32)

Asymmetric/Biological Unit(hide GO term definitions)

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  Cis Peptide Bonds
    Thr B:241 - Pro B:242   [ RasMol ]  
 

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        LT_SV40 | P03070: 1ejl 1gh6 1n25 1q1s 1q1t 1svl 1svm 1svo 1tbd 1z1d 2fuf 2h1l 2if9 2ipr 2itj 2itl 2nl8 2ntc 2tbd 3qk2 3qn2 4e2i 4fgn 4gdf 5d9i 5tct

(-) Related Entries Specified in the PDB File

1ejl MOUSE IMPORTIN ALPHA - SV40 NLS PEPTIDE COMPLEX