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(-) Description

Title :  AN ISOFORM-SPECIFIC MYRISTYLATION SWITCH TARGETS RIIB PKA HOLOENZYMES TO MEMBRANES
 
Authors :  P. Zhang, F. Ye, A. C. Bastidas, A. P. Kornev, M. H. Ginsberg, J. Wu, S. S. T
Date :  09 Dec 14  (Deposition) - 22 Jul 15  (Release) - 16 Sep 15  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.40
Chains :  Asym./Biol. Unit :  A,B
Keywords :  Pka, Membrane Binding, Molecular Switch, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  P. Zhang, F. Ye, A. C. Bastidas, A. P. Kornev, J. Wu, M. H. Ginsberg, S. S. Taylor
An Isoform-Specific Myristylation Switch Targets Type Ii Pk Holoenzymes To Membranes.
Structure V. 23 1563 2015
PubMed-ID: 26278174  |  Reference-DOI: 10.1016/J.STR.2015.07.007

(-) Compounds

Molecule 1 - CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT ALPHA
    Chains: A
    EC Number: 2.7.11.11
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Taxid: 562
    Gene: PRKACA, PKACA
    Mutation: YES
    Organism Common: MOUSE
    Organism Scientific: MUS MUSCULUS
    Organism Taxid: 10090
    Synonym: PKA C-ALPHA
 
Molecule 2 - CAMP-DEPENDENT PROTEIN KINASE TYPE I-ALPHA REGULATORY SUBUNIT
    Chains: B
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Taxid: 562
    Gene: PRKAR1A
    Mutation: YES
    Organism Common: BOVINE
    Organism Scientific: BOS TAURUS
    Organism Taxid: 9913

 Structural Features

(-) Chains, Units

  12
Asymmetric/Biological Unit : AB

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (8, 16)

Asymmetric/Biological Unit (8, 16)
No.NameCountTypeFull Name
1ATP1Ligand/IonADENOSINE-5'-TRIPHOSPHATE
2GOL2Ligand/IonGLYCEROL
3MG2Ligand/IonMAGNESIUM ION
4MYR1Ligand/IonMYRISTIC ACID
5SEP2Mod. Amino AcidPHOSPHOSERINE
6SO46Ligand/IonSULFATE ION
7TAM1Ligand/IonTRIS(HYDROXYETHYL)AMINOMETHANE
8TPO1Mod. Amino AcidPHOSPHOTHREONINE

(-) Sites  (13, 13)

Asymmetric Unit (13, 13)
No.NameEvidenceResiduesDescription
01AC1SOFTWAREARG A:137 , HOH A:539binding site for residue SO4 A 401
02AC2SOFTWAREGLY A:50 , THR A:51 , GLY A:52 , SER A:53 , PHE A:54 , GLY A:55 , VAL A:57 , ALA A:70 , LYS A:72 , MET A:120 , GLU A:121 , VAL A:123 , GLU A:127 , ASP A:166 , LYS A:168 , GLU A:170 , ASN A:171 , LEU A:173 , THR A:183 , ASP A:184 , PHE A:327 , MG A:407 , MG A:408 , ARG B:94 , ALA B:97binding site for residue ATP A 402
03AC3SOFTWARELYS A:189 , ARG A:194 , THR A:195 , TRP A:196 , TPO A:197 , TYR B:103 , THR B:104 , GLU B:105 , GLU B:106 , HOH B:610binding site for residue TAM A 403
04AC4SOFTWAREASP A:329 , TYR A:330 , HOH A:560 , HOH A:567 , LYS B:90binding site for residue GOL A 404
05AC5SOFTWAREGLY A:1 , ASN A:2 , PHE A:18 , LEU A:152 , GLU A:155 , GLN A:307 , LYS A:309binding site for residue MYR A 405
06AC6SOFTWAREARG A:194 , ASN A:216 , LYS A:217 , HOH A:561binding site for residue SO4 A 406
07AC7SOFTWAREASN A:171 , ASP A:184 , ATP A:402binding site for residue MG A 407
08AC8SOFTWAREPHE A:54 , ASP A:184 , ATP A:402binding site for residue MG A 408
09AC9SOFTWAREPHE B:322 , GLY B:323 , GLU B:324 , ILE B:325 , HOH B:548 , HOH B:550 , HOH B:551 , HOH B:579 , HOH B:582 , HOH B:619 , HOH B:621binding site for residue SO4 B 401
10AD1SOFTWAREPRO A:258 , SER A:259 , GLN B:283 , GLY B:284 , ARG B:303binding site for residue SO4 B 402
11AD2SOFTWAREILE B:163 , GLY B:169 , ASP B:170 , ASN B:171 , TYR B:173 , ARG B:209 , HOH B:567binding site for residue SO4 B 403
12AD3SOFTWAREARG B:93 , MET B:329 , ASN B:330 , ARG B:350 , HOH B:505 , HOH B:509binding site for residue SO4 B 404
13AD4SOFTWARELYS A:83 , SER B:99 , ALA B:100 , GLU B:101 , GLY B:206 , ARG B:226binding site for residue GOL B 405

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4X6R)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 4X6R)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4X6R)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4X6R)

(-) Exons   (0, 0)

(no "Exon" information available for 4X6R)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:350
                                                                                                                                                                                                                                                                                                                                                                                              
               SCOP domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ......hhhhhhhhhhhhhhhhhhhhhhhhhh......hhh.eeeeeeeee....eeeeeee.....eeeeeeeehhhhhhh.hhhhhhhhhhhhhhh.......eeeeee...eeeeeee.....hhhhhhhhhh..hhhhhhhhhhhhhhhhhhhhhh.ee....hhh.eee.....eee......ee........eehhhhhhhhhhh.....hhhhhhhhhhhhhhhhhh.......hhhhhhhhhhh.........hhhhhhhhhhhh..............hhhhhhhhhhh..hhhhhhh........................................... Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4x6r A   1 GNAAAACKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFsEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWtLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVsINEKCGKEFTEF 350
                                    10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190      |200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350
                                                                                                                                                                    139-SEP                                                   197-TPO                                                                                                                                      338-SEP        


Chain B from PDB  Type:PROTEIN  Length:290
                                                                                                                                                                                                                                                                                                                                  
               SCOP domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ........ee....hhhhhhh........hhhhhhhhhhhh..hhhhhh.hhhhhhhhhhhheeeee....eee.......eeeeeee..eeeee..eeeeee....eehhhhhh.......eeee...eeeeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhh..hhhhhhhhhhhheeeee....eee.......eeeeeeeeeeeeeee......eeeeeee....ee.hhhhhh......eeeeeeeeeeeeeehhhhhhhhhhhhhhhh.hhhhhhhhh.... Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 4x6r B  90 KGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPKAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV 379
                                    99       109       119       129       139       149       159       169       179       189       199       209       219       229       239       249       259       269       279       289       299       309       319       329       339       349       359       369       379
   Legend:   → Mismatch (orange background)
  - → Gap (green background, '-', border residues have a numbering label)
    → Modified Residue (blue background, lower-case, 'x' indicates undefined single-letter code, labelled with number + name)
  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4X6R)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4X6R)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4X6R)

(-) Gene Ontology  (73, 84)

Asymmetric/Biological Unit(hide GO term definitions)

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  KAPCA_MOUSE | P05132
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  2.7.11.11
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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        KAP0_BOVIN | P00514: 1apk 1bpk 1ne4 1ne6 1pvk 1rgs 1rl3 2ezw 2qcs 3fhi 3iia 3im3 3im4 3plq 3pna 3pvb 4jv4 4mx3 5hvz 5jr7
        KAPCA_MOUSE | P05132: 1apm 1atp 1bkx 1bx6 1fmo 1j3h 1jbp 1jlu 1l3r 1pvk 1rdq 1re8 1rej 1rek 1syk 2cpk 2erz 2qcs 2qur 2qvs 3fhi 3fjq 3idb 3idc 3j4q 3j4r 3o7l 3ow3 3pvb 3qal 3qam 3tnp 3tnq 3x2u 3x2v 3x2w 4dfx 4dfy 4dfz 4dg0 4dg2 4dg3 4dh1 4dh3 4dh5 4dh7 4dh8 4din 4hpt 4hpu 4iac 4iad 4iaf 4iai 4iak 4iay 4iaz 4ib0 4ib1 4ib3 4nts 4ntt 4o21 4o22 4wbb 4x6q 4xw4 4xw5 4xw6 5jr7 5x3f

(-) Related Entries Specified in the PDB File

4x6q