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(-) Description

Title :  CRYSTAL STRUCTURE OF PROTEINASE K PROCESSED WITH THE CRYSTALDIRECT AUTOMATED MOUNTING AND CRYO-COOLING TECHNOLOGY
 
Authors :  U. Zander, G. Hoffmann, I. Cornaciu, F. Cipriani, J. A. Marquez
Date :  02 Sep 15  (Deposition) - 13 Apr 16  (Release) - 20 Apr 16  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.01
Chains :  Asym./Biol. Unit :  A
Keywords :  Hydrolase, Proteinase, Automated Crystal Harvesting, Automated Cryo- Cooling, Crystaldirect (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  U. Zander, G. Hoffmann, I. Cornaciu, J. -P. Marquette, G. Papp, C. Landret, G. Seroul, J. Sinoir, M. Roewer, F. Felisaz, S. Rodriguez-Puente, V. Mariaule, P. Murphy, M. Mathieu, F. Cipriani J. A. Marquez
Automated Harvesting And Processing Of Protein Crystals Through Laser Photoablation.
Acta Crystallogr. , Sect. D V. 72 454 2016
PubMed-ID: 27050125  |  Reference-DOI: 10.1107/S2059798316000954

(-) Compounds

Molecule 1 - PROTEINASE K
    Chains: A
    EC Number: 3.4.21.64
    Fragment: UNP RESIDUES 106-384
    Organism Scientific: ENGYODONTIUM ALBUM
    Organism Taxid: 37998
    Synonym: ENDOPEPTIDASE K, TRITIRACHIUM ALKALINE PROTEINASE

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit : A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 1)

Asymmetric/Biological Unit (1, 1)
No.NameCountTypeFull Name
1SO41Ligand/IonSULFATE ION

(-) Sites  (1, 1)

Asymmetric Unit (1, 1)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREPRO A:7 , TRP A:8 , ARG A:185 , HOH A:2011 , HOH A:2276 , HOH A:2277 , HOH A:2357 , HOH A:2359BINDING SITE FOR RESIDUE SO4 A1280

(-) SS Bonds  (2, 2)

Asymmetric/Biological Unit
No.Residues
1A:34 -A:123
2A:178 -A:249

(-) Cis Peptide Bonds  (1, 1)

Asymmetric/Biological Unit
No.Residues
1Ser A:170 -Pro A:171

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5AMX)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5AMX)

(-) Exons   (0, 0)

(no "Exon" information available for 5AMX)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:279
                                                                                                                                                                                                                                                                                                                       
               SCOP domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .ee...hhhhhhhh........ee........eeeeeee......hhhhh..eeeeee.........hhhhhhhhhhhh.........eeeeee........hhhhhhhhhhhhhhhhhhh....eeeeee...ee.hhhhhhhhhhhhhh.eeeeee..........ee.......eeeeee.................eeee...eeeee...eeeee.hhhhhhhhhhhhhhhhhhh......hhhhhhhhhhee............ee....... Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5amx A   1 AAQTNAPWGLARISSTSPGTSTYYYDESAGQGSCVYVIDTGIEASHPEFEGRAQMVKTYYYSSRDGNGHGTHCAGTVGSRTYGVAKKTQLFGVKVLDDNGSGQYSTIIAGMDFVASDKNNRNCPKGVVASLSLGGGYSSSVNSAAARLQSSGVMVAVAAGNNNADARNYSPASEPSVCTVGASDRYDRRSSFSNYGSVLDIFGPGTSILSTWIGGSTRSISGTSMATPHVAGLAAYLMTLGKTTAASACRYIADTANKGDLSNIPFGTVNLLAYNNYQA 279
                                    10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270
   Legend:   → Mismatch (orange background)
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  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5AMX)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5AMX)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5AMX)

(-) Gene Ontology  (6, 6)

Asymmetric/Biological Unit(hide GO term definitions)

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  PRTK_PARAQ | P06873
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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        PRTK_PARAQ | P06873: 1bjr 1cnm 1egq 1ht3 1ic6 1oyo 1p7v 1p7w 1pek 1pfg 1pj8 1ptk 2dp4 2dqk 2duj 2g4v 2hd4 2hpz 2id8 2pkc 2pq2 2prk 2pwa 2pwb 2pyz 2v8b 3aj8 3aj9 3d9q 3ddz 3de0 3de1 3de2 3de3 3de4 3de5 3de6 3de7 3dvq 3dvr 3dvs 3dw1 3dw3 3dwe 3dyb 3gt3 3gt4 3i2y 3i30 3i34 3i37 3l1k 3osz 3prk 3ptl 3q40 3q5g 3qmp 4b5l 4dj5 4fon 4wob 4woc 4zar 5avj 5avk 5b1d 5b1e 5cw1 5i9s 5k7s 5kxu 5kxv 5uvl

(-) Related Entries Specified in the PDB File

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