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(-) Description

Title :  CRYSTAL STRUCTURE OF UBCH5B IN COMPLEX WITH THE RING-U5BR FRAGMENT OF AO7 (Y165A)
 
Authors :  Y. -H. Liang, S. Li, A. M. Weissman, X. Ji
Date :  08 Oct 13  (Deposition) - 28 Oct 15  (Release) - 30 Dec 15  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.62
Chains :  Asym./Biol. Unit :  A,B
Keywords :  Ubiquitin Ligase, Ubiquitin Conjugating Enzyme, Ubiquitylation, Ubiquitin, Ligase (Keyword Search: [Gene Ontology, PubMed, Web (Google))
 
Reference :  S. Li, Y. H. Liang, J. Mariano, M. B. Metzger, D. K. Stringer, V. A. Hristova, J. Li, P. A. Randazzo, Y. C. Tsai, X. Ji, A. M. Weissman
Insights Into Ubiquitination From The Unique Clamp-Like Binding Of The Ring E3 Ao7 To The E2 Ubch5B.
J. Biol. Chem. V. 290 30225 2015
PubMed-ID: 26475854  |  Reference-DOI: 10.1074/JBC.M115.685867

(-) Compounds

Molecule 1 - UBIQUITIN-CONJUGATING ENZYME E2 D2
    ChainsA
    EC Number6.3.2.19
    EngineeredYES
    Expression SystemESCHERICHIA COLI
    Expression System PlasmidPETDUET
    Expression System StrainBL21 (DE3)
    Expression System Taxid469008
    Expression System Vector TypePLASMID
    GeneUBE2D2, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B
    Organism CommonHUMAN
    Organism ScientificHOMO SAPIENS
    Organism Taxid9606
    SynonymUBIQUITIN CARRIER PROTEIN D2,UBIQUITIN-CONJUGATING ENZYME E2(17)KB 2,UBIQUITIN-CONJUGATING ENZYME E2-17 KDA 2,UBIQUITIN-PROTEIN LIGASE D2,P53-REGULATED UBIQUITIN-CONJUGATING ENZYME 1
 
Molecule 2 - E3 UBIQUITIN-PROTEIN LIGASE RNF25
    ChainsB
    EC Number6.3.2.-
    EngineeredYES
    Expression SystemESCHERICHIA COLI
    Expression System PlasmidPETDUET
    Expression System StrainBL21 (DE3)
    Expression System Taxid469008
    Expression System Vector TypePLASMID
    FragmentUNP RESIDUES 126-258
    GeneRNF25
    MutationYES
    Organism CommonHUMAN
    Organism ScientificHOMO SAPIENS
    Organism Taxid9606
    SynonymRING FINGER PROTEIN 25

 Structural Features

(-) Chains, Units

  12
Asymmetric/Biological Unit AB

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (3, 4)

Asymmetric/Biological Unit (3, 4)
No.NameCountTypeFull Name
1OXL1Ligand/IonOXALATE ION
2PEG1Ligand/IonDI(HYDROXYETHYL)ETHER
3ZN2Ligand/IonZINC ION

(-) Sites  (4, 4)

Asymmetric Unit (4, 4)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREARG A:72 , TRP A:141 , TYR A:145 , OXL A:402 , HOH A:570 , HOH A:613binding site for residue PEG A 401
2AC2SOFTWARETRP A:141 , LYS A:144 , TYR A:145 , PEG A:401binding site for residue OXL A 402
3AC3SOFTWARECYS B:135 , CYS B:138 , HIS B:158 , CYS B:161binding site for residue ZN B 301
4AC4SOFTWARECYS B:153 , HIS B:155 , CYS B:198 , CYS B:201binding site for residue ZN B 302

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 5D1L)

(-) Cis Peptide Bonds  (1, 1)

Asymmetric/Biological Unit
No.Residues
1Tyr A:60 -Pro A:61

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5D1L)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5D1L)

(-) Exons   (0, 0)

(no "Exon" information available for 5D1L)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it)
Chain A from PDB  Type:PROTEIN  Length:146
                                                                                                                                                                                  
               SCOP domains -------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author hhhhhhhhhhhhhhh....eeeeee..eeeeeeeeee..........eeeeeee..........eeee................hhhhh.......hhhhhhhhhhhhhhh.......hhhhhhhhhhhhhhhhhhhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5d1l A   2 ALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147
                                    11        21        31        41        51        61        71        81        91       101       111       121       131       141      

Chain B from PDB  Type:PROTEIN  Length:111
                                                                                                                                               
               SCOP domains --------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ...................eee.....eeehhhhhhhhhhhhhhhhhhh..............hhhhhhhh............hhhhhhhhhhhhhhhhhhhhh....... Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------------------------- Transcript
                 5d1l B 129 NIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARAIQHMEQELKAQGVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLE 255
                                   138       148       158       168       194       204       214       224       234       244       254 
                                                                          177|                                                             
                                                                           194                                                             

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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5D1L)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5D1L)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5D1L)

(-) Gene Ontology  (29, 32)

Asymmetric/Biological Unit(hide GO term definitions)

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    Tyr A:60 - Pro A:61   [ RasMol ]  
 

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        RNF25_HUMAN | Q96BH12day 2dmf 5d1k 5d1m
        UB2D2_HUMAN | P628371ur6 1w4u 2clw 2esk 2eso 2esp 2esq 3a33 3jvz 3jw0 3l1y 3tgd 3zni 4a49 4a4b 4a4c 4auq 4ddg 4ddi 4ldt 4v3k 4v3l 4wz3 5d0k 5d0m 5d1k 5d1m 5edv 5mnj 5ulf 5ulh 5ulk

(-) Related Entries Specified in the PDB File

5d1k 5d1m