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(-) Description

Title :  CRYSTAL STRUCTURE OF THE DNA BINDING DOMAIN OF HUMAN TRANSCRIPTION FACTOR FLI1 IN COMPLEX WITH A 10-MER DNA ACCGGAAGTG
 
Authors :  C. Hou, O. V. Tsodikov
Date :  14 Oct 15  (Deposition) - 09 Dec 15  (Release) - 30 Dec 15  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  3.45
Chains :  Asym. Unit :  A,B,C,D,E,F,G,H,I,J,K,L
Biol. Unit 1:  A,B,C,G,H,I  (1x)
Biol. Unit 2:  D,E,F,J,K,L  (1x)
Keywords :  Transcription, Dna Binding, Ewing Sarcoma, Winged Helix, Ets Family, Dna Binding Protein-Dna Complex (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  C. Hou, O. V. Tsodikov
Structural Basis For Dimerization And Dna Binding Of Transcription Factor Fli1.
Biochemistry V. 54 7365 2015
PubMed-ID: 26618620  |  Reference-DOI: 10.1021/ACS.BIOCHEM.5B01121

(-) Compounds

Molecule 1 - FRIEND LEUKEMIA INTEGRATION 1 TRANSCRIPTION FACTOR
    Chains: A, D, G, J
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Strain: BL21(DE3)
    Expression System Taxid: 469008
    Expression System Vector Type: PLASMID
    Fragment: UNP RESIDUES 276-399
    Gene: FLI1
    Organism Common: HUMAN
    Organism Scientific: HOMO SAPIENS
    Organism Taxid: 9606
    Synonym: PROTO-ONCOGENE FLI-1,TRANSCRIPTION FACTOR ERGB
 
Molecule 2 - DNA (5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3')
    Chains: B, E, H, K
    Engineered: YES
    Organism Scientific: ENDOTHIA GYROSA
    Organism Taxid: 40263
    Synthetic: YES
 
Molecule 3 - DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3')
    Chains: C, F, I, L
    Engineered: YES
    Organism Scientific: ENDOTHIA GYROSA
    Organism Taxid: 40263
    Synthetic: YES

 Structural Features

(-) Chains, Units

  123456789101112
Asymmetric Unit : ABCDEFGHIJKL
Biological Unit 1 (1x): ABC   GHI   
Biological Unit 2 (1x):    DEF   JKL

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 8)

Asymmetric Unit (1, 8)
No.NameCountTypeFull Name
1CA8Ligand/IonCALCIUM ION
Biological Unit 1 (0, 0)
No.NameCountTypeFull Name
1CA-1Ligand/IonCALCIUM ION
Biological Unit 2 (0, 0)
No.NameCountTypeFull Name
1CA-1Ligand/IonCALCIUM ION

(-) Sites  (3, 3)

Asymmetric Unit (3, 3)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREDG B:9binding site for residue CA B 101
2AC2SOFTWAREDG C:21binding site for residue CA C 101
3AC3SOFTWAREDG F:21binding site for residue CA F 101

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 5E8I)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 5E8I)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5E8I)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5E8I)

(-) Exons   (0, 0)

(no "Exon" information available for 5E8I)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:93
                                                                                                                             
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ...hhhhhhhhhhhhhhhhh.eee.....eeee.hhhhhhhhhhhhhh....hhhhhhhhhhhhhhh..eee......eeeehhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                 5e8i A 279 GQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQP 371
                                   288       298       308       318       328       338       348       358       368   


Chain B from PDB  Type:DNA  Length:10
                                          
                 5e8i B   2 ACCGGAAGTG  11
                                    11


Chain C from PDB  Type:DNA  Length:10
                                          
                 5e8i C  14 CACTTCCGGT  23
                                    23


Chain D from PDB  Type:PROTEIN  Length:93
                                                                                                                             
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ...hhhhhhhhhhhhhhhhhh.ee.....eee..hhhhhhhhhhhhhh....hhhhhhhhhhhhhhh..eee......eeeehhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                 5e8i D 279 GQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQP 371
                                   288       298       308       318       328       338       348       358       368   


Chain E from PDB  Type:DNA  Length:10
                                          
                 5e8i E   2 ACCGGAAGTG  11
                                    11


Chain F from PDB  Type:DNA  Length:10
                                          
                 5e8i F  14 CACTTCCGGT  23
                                    23


Chain G from PDB  Type:PROTEIN  Length:93
                                                                                                                             
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ...hhhhhhhhhhhhhhhh...ee.....eee..hhhhhhhhhhhhhh....hhhhhhhhhhh......eee......eeeehhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                 5e8i G 279 GQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQP 371
                                   288       298       308       318       328       338       348       358       368   


Chain H from PDB  Type:DNA  Length:10
                                          
                 5e8i H   2 ACCGGAAGTG  11
                                    11


Chain I from PDB  Type:DNA  Length:10
                                          
                 5e8i I  14 CACTTCCGGT  23
                                    23


Chain J from PDB  Type:PROTEIN  Length:93
                                                                                                                             
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ...hhhhhhhhhhhhhhhh...ee.....eee..hhhhhhhhhhhhhh....hhhhhhhhhhhh.....eee......eeeehhhhhhhhhh. Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                 5e8i J 279 GQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQP 371
                                   288       298       308       318       328       338       348       358       368   


Chain K from PDB  Type:DNA  Length:10
                                          
                 5e8i K   2 ACCGGAAGTG  11
                                    11


Chain L from PDB  Type:DNA  Length:10
                                          
                 5e8i L  14 CACTTCCGGT  23
                                    23
   Legend:   → Mismatch (orange background)
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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5E8I)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5E8I)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5E8I)

(-) Gene Ontology  (20, 20)

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 Related Entries

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UniProtKB/Swiss-Prot
        FLI1_HUMAN | Q01543: 1fli 1x66 2ytu 5e8g 5jvt

(-) Related Entries Specified in the PDB File

5e8g