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(-) Description

Title :  BOVINE HEART 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE (PFKFB2)
 
Authors :  R. B. Crochet
Date :  22 Jan 16  (Deposition) - 16 Nov 16  (Release) - 28 Dec 16  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.82
Chains :  Asym./Biol. Unit :  A
Keywords :  Bovine Pfkfb2, Hydrolase, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  R. B. Crochet, J. D. Kim, H. Lee, Y. S. Yim, S. G. Kim, D. Neau, Y. H. Lee
Crystal Structure Of Heart 6-Phosphofructo-2-Kinase/Fructose-2, 6-Bisphosphatase (Pfkfb2) And The Inhibitory Influence Of Citrate On Substrate Binding.
Proteins V. 85 117 2017
PubMed-ID: 27802586  |  Reference-DOI: 10.1002/PROT.25204

(-) Compounds

Molecule 1 - 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE 2
    Chains: A
    EC Number: 2.7.1.105, 3.1.3.46
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PET3A
    Expression System Strain: BL21
    Expression System Taxid: 469008
    Expression System Variant: DE3
    Expression System Vector Type: PLASMID
    Gene: PFKFB2
    Organism Common: BOVINE
    Organism Scientific: BOS TAURUS
    Organism Taxid: 9913
    Synonym: PFK/FBPASE 2,6PF-2-K/FRU-2,6-P2ASE HEART-TYPE ISOZYME
    Tissue: HEART

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit : A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (5, 5)

Asymmetric/Biological Unit (5, 5)
No.NameCountTypeFull Name
1ADP1Ligand/IonADENOSINE-5'-DIPHOSPHATE
2DIO1Ligand/Ion1,4-DIETHYLENE DIOXIDE
3F6P1Ligand/IonFRUCTOSE-6-PHOSPHATE
4FLC1Ligand/IonCITRATE ANION
5NEP1Mod. Amino AcidN1-PHOSPHONOHISTIDINE

(-) Sites  (4, 4)

Asymmetric Unit (4, 4)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREPHE A:92 , HIS A:94 , ARG A:103 , CYS A:197 , TYR A:198 , THR A:201binding site for residue DIO A 601
2AC2SOFTWARENEP A:258 , ILE A:269 , GLY A:270 , GLU A:327 , TYR A:338 , ARG A:352 , LYS A:356 , TYR A:367 , GLN A:393 , ARG A:397 , HOH A:754 , HOH A:764 , HOH A:795 , HOH A:802 , HOH A:832binding site for residue F6P A 602
3AC3SOFTWARELEU A:75 , GLY A:76 , ARG A:79 , PHE A:92 , ARG A:103 , ALA A:130 , THR A:131 , TYR A:198 , HOH A:714 , HOH A:717 , HOH A:718 , HOH A:728 , HOH A:877 , HOH A:930 , HOH A:994binding site for residue FLC A 603
4AC4SOFTWAREALA A:49 , ARG A:50 , GLY A:51 , LYS A:52 , THR A:53 , TYR A:54 , SER A:157 , ASN A:168 , GLU A:171 , VAL A:172 , LYS A:173 , VAL A:219 , VAL A:248 , TYR A:429 , HOH A:718 , HOH A:728 , HOH A:805 , HOH A:849 , HOH A:989 , HOH A:1011 , HOH A:1013binding site for residue ADP A 604

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 5HR5)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 5HR5)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5HR5)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5HR5)

(-) Exons   (0, 0)

(no "Exon" information available for 5HR5)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
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Chain A from PDB  Type:PROTEIN  Length:424
                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
               SCOP domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ..hhhhhh....eeeeee.....hhhhhhhhhhhhhhhh...eeeeehhhhhhhhhh...hhhhhh..hhhhhhhhhhhhhhhhhhhhhhhhh....eeeee....hhhhhhhhhhhhhhh..eeeeeeee..hhhhhhhhhhhhh.........hhhhhhhhhhhhhhhhhhhh.............eeeee....eeeee...hhhhhhhhhhhh........eeeee...hhhhhhh........hhhhhhhhhhhhhhhhhhh....eeee..hhhhhhhhhh.....ee.hhhh...hhhhh..hhhhhhhhhhhhhhhhhhh..........hhhhhhhhhhhhhhhhhhh..eeeeehhhhhhhhhhhhhh....hhhhh.....eeeeeeee..eeeeeeee.............. Sec.struct. author
                 SAPs(SNPs) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5hr5 A  28 KCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRRQAVKSYKSYDFFRHDNEEAMKIRKQCALVALKDVKAYLTEESGQIAVFDATNTTRERRDLILNFAEENSFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMDDFLKRIECYKVTYQPLDPDSHDKDLSFIKVINVGQRFLVNKVQDYIQSKIVYYLMNIHVHPRTIYLCRhGESEFNLLGKIGGDSGLSVRGKQFAQALRKFLEEQEIADLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYAEIQEQYPDEFALRDEEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEAVNTHRDKPT 451
                                    37        47        57        67        77        87        97       107       117       127       137       147       157       167       177       187       197       207       217       227       237       247       257|      267       277       287       297       307       317       327       337       347       357       367       377       387       397       407       417       427       437       447    
                                                                                                                                                                                                                                                                258-NEP
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 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5HR5)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5HR5)

(-) Pfam Domains  (0, 0)

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(-) Gene Ontology  (16, 16)

Asymmetric/Biological Unit(hide GO term definitions)

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5htk