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(-) Description

Title :  HUMAN L-TYPE FERRITIN IRON LOADED FOR 60 MINUTES
 
Authors :  C. Pozzi, F. Di Pisa, S. Mangani
Date :  06 Jul 16  (Deposition) - 22 Feb 17  (Release) - 22 Mar 17  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.98
Chains :  Asym. Unit :  A
Biol. Unit 1:  A  (24x)
Keywords :  L-Type, Ferritin, Mineralization, Iron, Metal Binding Protein (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  C. Pozzi, S. Ciambellotti, C. Bernacchioni, F. Di Pisa, S. Mangani, P. Turano
Chemistry At The Protein-Mineral Interface In L-Ferritin Assists The Assembly Of A Functional ( Mu (3)-Oxo)Tris[( Mu (2)-Peroxo)] Triiron(Iii) Cluster.
Proc. Natl. Acad. Sci. V. 114 2580 2017 U. S. A.
PubMed-ID: 28202724  |  Reference-DOI: 10.1073/PNAS.1614302114

(-) Compounds

Molecule 1 - FERRITIN LIGHT CHAIN
    Chains: A
    Engineered: YES
    Expression System: ESCHERICHIA COLI BL21
    Expression System Plasmid: PET21C
    Expression System Taxid: 511693
    Expression System Variant: PLYSS
    Expression System Vector Type: PLASMID
    Gene: FTL
    Organism Common: HUMAN
    Organism Scientific: HOMO SAPIENS
    Organism Taxid: 9606
    Synonym: FERRITIN L SUBUNIT

 Structural Features

(-) Chains, Units

  1
Asymmetric Unit : A
Biological Unit 1 (24x): A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (5, 17)

Asymmetric Unit (5, 17)
No.NameCountTypeFull Name
1CD8Ligand/IonCADMIUM ION
2FE4Ligand/IonFE (III) ION
3OXY1Ligand/IonOXYGEN MOLECULE
4PER3Ligand/IonPEROXIDE ION
5SO41Ligand/IonSULFATE ION
Biological Unit 1 (3, 120)
No.NameCountTypeFull Name
1CD-1Ligand/IonCADMIUM ION
2FE-1Ligand/IonFE (III) ION
3OXY24Ligand/IonOXYGEN MOLECULE
4PER72Ligand/IonPEROXIDE ION
5SO424Ligand/IonSULFATE ION

(-) Sites  (17, 17)

Asymmetric Unit (17, 17)
No.NameEvidenceResiduesDescription
01AC1SOFTWAREASP A:15 , HOH A:432 , HOH A:445binding site for residue CD A 201
02AC2SOFTWAREASP A:131binding site for residue CD A 202
03AC3SOFTWARECYS A:130 , GLU A:134binding site for residue CD A 203
04AC4SOFTWAREHIS A:118 , CYS A:130 , GLU A:134 , HOH A:484binding site for residue CD A 204
05AC5SOFTWAREHIS A:136 , HOH A:449 , HOH A:464binding site for residue CD A 205
06AC6SOFTWAREGLU A:90 , HOH A:395 , HOH A:416 , HOH A:450binding site for residue CD A 206
07AC7SOFTWAREGLU A:49 , ASP A:178binding site for residue CD A 207
08AC8SOFTWAREGLU A:90binding site for residue CD A 208
09AC9SOFTWAREGLU A:61 , GLU A:64 , FE A:210 , FE A:211 , OXY A:213 , PER A:214 , PER A:216 , HOH A:383binding site for residue FE A 209
10AD1SOFTWAREGLU A:57 , GLU A:60 , GLU A:61 , FE A:209 , FE A:211 , OXY A:213 , PER A:215 , PER A:216binding site for residue FE A 210
11AD2SOFTWAREGLU A:60 , GLU A:64 , FE A:209 , FE A:210 , OXY A:213 , PER A:214 , PER A:215 , HOH A:382binding site for residue FE A 211
12AD3SOFTWAREGLU A:57 , PER A:215 , HOH A:457 , HOH A:496binding site for residue FE A 212
13AD4SOFTWAREGLU A:60 , GLU A:61 , GLU A:64 , FE A:209 , FE A:210 , FE A:211 , PER A:214 , PER A:215 , PER A:216binding site for residue OXY A 213
14AD5SOFTWAREGLU A:64 , FE A:209 , FE A:211 , OXY A:213 , PER A:216 , HOH A:383binding site for residue PER A 214
15AD6SOFTWAREGLU A:57 , GLU A:60 , FE A:210 , FE A:211 , FE A:212 , OXY A:213 , HOH A:388binding site for residue PER A 215
16AD7SOFTWAREGLU A:57 , GLU A:61 , FE A:209 , FE A:210 , OXY A:213 , PER A:214binding site for residue PER A 216
17AD8SOFTWARESER A:13 , ASP A:15 , ARG A:124 , HOH A:318 , HOH A:327 , HOH A:364 , HOH A:415 , HOH A:432binding site for residue SO4 A 217

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 5LG8)

(-) Cis Peptide Bonds  (0, 0)

(no "Cis Peptide Bond" information available for 5LG8)

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5LG8)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5LG8)

(-) Exons   (0, 0)

(no "Exon" information available for 5LG8)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
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SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:173
                                                                                                                                                                                                             
               SCOP domains ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author .......hhhhhhhhhhhhhhhhhhhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhhhhhhhhhhh.................hhhhhhhhhhhhhhhhhhhhhhhhhhhhhh.hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh.... Sec.struct. author
                 SAPs(SNPs) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5lg8 A   6 SQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD 178
                                    15        25        35        45        55        65        75        85        95       105       115       125       135       145       155       165       175
   Legend:   → Mismatch (orange background)
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  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5LG8)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5LG8)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5LG8)

(-) Gene Ontology  (12, 12)

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 Related Entries

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        FRIL_HUMAN | P02792: 2ffx 2fg4 2fg8 3kxu 4v6b

(-) Related Entries Specified in the PDB File

5lg2 HORSE L-TYPE FERRITIN IRON LOADED FOR 60 MINUTES