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(-) Description

Title :  CRYSTAL STRUCTURE OF A LACCASE-LIKE MULTICOPPER OXIDASE MCOG FROM FROM ASPERGILLUS NIGER
 
Authors :  M. Ferraroni, F. Briganti, J. A. Tamayo-Ramos, W. J. H. Van Berkel, A. H
Date :  29 Jul 16  (Deposition) - 03 May 17  (Release) - 17 May 17  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.70
Chains :  Asym./Biol. Unit :  A
Keywords :  Oxidoreductase (Keyword Search: [Gene Ontology, PubMed, Web (Google))
 
Reference :  M. Ferraroni, A. H. Westphal, M. Borsari, J. A. Tamayo-Ramos, F. Briganti, L. H. De Graaff, W. J. H. Van Berkel
Structure And Function Of Aspergillus Niger Laccase Mcog
Biocatalysis 2017
PubMed: search  |  Reference-DOI: 10.1515/BOCA-2017-0001

(-) Compounds

Molecule 1 - 'MULTICOPPER OXIDASE
    ChainsA
    EC Number1.10.3.2
    EngineeredYES
    Expression SystemESCHERICHIA COLI
    Expression System PlasmidPALIV
    Expression System Taxid562
    Expression System Vector TypePLASMID
    GeneAN08G08450
    Organism ScientificASPERGILLUS NIGER (STRAIN CBS 513.88 / FGSC A1513)
    Organism Taxid425011

 Structural Features

(-) Chains, Units

  1
Asymmetric/Biological Unit A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (5, 18)

Asymmetric/Biological Unit (5, 18)
No.NameCountTypeFull Name
1BMA2Ligand/IonBETA-D-MANNOSE
2CU4Ligand/IonCOPPER (II) ION
3MAN2Ligand/IonALPHA-D-MANNOSE
4NAG9Ligand/IonN-ACETYL-D-GLUCOSAMINE
5PER1Ligand/IonPEROXIDE ION

(-) Sites  (11, 11)

Asymmetric Unit (11, 11)
No.NameEvidenceResiduesDescription
01AC1SOFTWAREHIS A:110 , HIS A:152 , HIS A:517 , PER A:618binding site for residue CU A 601
02AC2SOFTWAREHIS A:154 , HIS A:451 , HIS A:453 , HIS A:515 , PER A:618binding site for residue CU A 602
03AC3SOFTWAREHIS A:108 , HIS A:110 , HIS A:451 , HIS A:453 , PER A:618 , HOH A:849binding site for residue CU A 603
04AC4SOFTWAREHIS A:448 , CYS A:516 , ILE A:518 , HIS A:521binding site for residue CU A 604
05AC5SOFTWAREASN A:134 , HOH A:729 , HOH A:1144binding site for residue NAG A 617
06AC6SOFTWAREHIS A:108 , HIS A:110 , HIS A:152 , HIS A:154 , HIS A:451 , HIS A:453 , HIS A:515 , HIS A:517 , CU A:601 , CU A:602 , CU A:603 , HOH A:833binding site for residue PER A 618
07AC7SOFTWAREASN A:60 , LEU A:73 , GLU A:101 , ASN A:102 , ASN A:103 , NAG A:608 , HOH A:701 , HOH A:711 , HOH A:730 , HOH A:874 , HOH A:927 , HOH A:937binding site for Poly-Saccharide residues NAG A 605 through NAG A 606 bound to ASN A 60
08AC8SOFTWARETHR A:83 , LEU A:181 , MET A:183 , ASN A:216 , NAG A:615 , HOH A:799 , HOH A:813 , HOH A:886 , HOH A:951binding site for Mono-Saccharide NAG A 614 bound to ASN A 216
09AC9SOFTWAREASN A:400 , ARG A:567 , LEU A:569 , HOH A:792 , HOH A:839 , HOH A:843 , HOH A:961binding site for Poly-Saccharide residues NAG A 612 through NAG A 613 bound to ASN A 400
10AD1SOFTWAREASN A:102 , ASN A:103 , SER A:156 , THR A:193 , ASP A:195 , GLU A:196 , TYR A:562 , GLU A:574 , NAG A:605 , HOH A:738 , HOH A:780 , HOH A:794 , HOH A:823 , HOH A:846 , HOH A:913 , HOH A:935 , HOH A:950 , HOH A:965 , HOH A:979 , HOH A:989 , HOH A:1075 , HOH A:1121binding site for Poly-Saccharide residues BMA A 607 through MAN A 611
11AD2SOFTWAREPRO A:82 , THR A:83 , GLU A:85 , ASN A:102 , ASN A:103 , SER A:156 , LEU A:168 , GLY A:182 , THR A:193 , ASP A:195 , GLU A:196 , TYR A:562 , GLU A:574 , NAG A:605 , MAN A:611 , NAG A:614 , HOH A:723 , HOH A:738 , HOH A:754 , HOH A:780 , HOH A:794 , HOH A:823 , HOH A:846 , HOH A:878 , HOH A:892 , HOH A:913 , HOH A:935 , HOH A:950 , HOH A:963 , HOH A:965 , HOH A:976 , HOH A:979 , HOH A:989 , HOH A:1019 , HOH A:1052 , HOH A:1075 , HOH A:1121binding site for Poly-Saccharide residues NAG A 615 through BMA A 616

(-) SS Bonds  (2, 2)

Asymmetric/Biological Unit
No.Residues
1A:129 -A:550
2A:314 -A:351

(-) Cis Peptide Bonds  (2, 2)

Asymmetric/Biological Unit
No.Residues
1Phe A:79 -Pro A:80
2Val A:387 -Pro A:388

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5LM8)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5LM8)

(-) Exons   (0, 0)

(no "Exon" information available for 5LM8)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it)
Chain A from PDB  Type:PROTEIN  Length:549
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
               SCOP domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ......................eeeeeeeeeeeee......eeeeee.....eeeeeee...eeeeeeeee.......eeee......hhhhh..............eeeeeee....eeeeeee...hhhhhh.eeeeeee........eeeeeeeeeee...hhhhhhhhhhh........eeee...eeee.....eeee..eeee....eeeeeeee......eeeee....eeeeee..eeeeeeee..eee....eeeeeee......eeeeeeee.............eeeeee...........................................eeeeeeeeeeee....eeeeee...........hhhhhhhhh......hhhh.eeee.....eeeeeeee.....eeeee....eeeeeeee................ee.eeee...hhhhh..eeeeeeee....eeeeeee.hhhhhhh..eeeeee.hhhhhhh.hhhhhhhhhhhhhhhhhhhhhhh............. Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5lm8 A  29 NTPWQGYDINTNYYETIPQTNVVREYWFDIVNTTAALDGVERPVLLVNGQFPGPTIEANWGDTVKVHVTNRMENNGTAIHFHGIRQLYNNQMDGVAALTQCPVPPNSSYTYVWRAEEYGSSWYHSHFSLQAWEGVFGGILIHGPSTAEYDHDLGMVFLNDWSHQTVDEMYQSVLESQNPPHFQTGLINGSNIWVTADNQTVGRRFQTEFVPGQRYRLRLVNAAMHTHFRFSIDNHDLTVIASDFVPIVPFTTNNVPIGMGQRYDIIVTANQAPDNYWIRAIPQSFCSDNANSDNIKGVLHYEGAADNSDPTSTKWDYGDDIQCLDFSLDELVPWLALDADIGGAQMAESDVDFTPFGDVPLYLWTMGGNALNISWKDPTLQQTFEDPDKMDWKASQGVIEAAIPNKWTVLVVQTDLPVPHPIHLHGHDFYLLAQGFGQFNPQNVTLKTHNPPRRDTALMTAATPENGGGGYMVIGFPADNPGVWLIHCHIGFHATEGFAQQIVERQSEFNTFFSEDLLENTCDAWDEYAKVNPYGHQYRALAGPYESGI 577
                                    38        48        58        68        78        88        98       108       118       128       138       148       158       168       178       188       198       208       218       228       238       248       258       268       278       288       298       308       318       328       338       348       358       368       378       388       398       408       418       428       438       448       458       468       478       488       498       508       518       528       538       548       558       568         

   Legend:   → Mismatch (orange background)
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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5LM8)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5LM8)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5LM8)

(-) Gene Ontology  (5, 5)

Asymmetric/Biological Unit(hide GO term definitions)

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        A2QS62_ASPNC | A2QS625lww 5lwx

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