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(-) Description

Title :  PROTEIN STRUCTURE OF TYPE III PLASMID SEGREGATION TUBR
 
Authors :  M. A. Schumacher, L. Ni
Date :  21 Mar 10  (Deposition) - 07 Jul 10  (Release) - 21 Jul 10  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.50
Chains :  Asym. Unit :  A
Biol. Unit 1:  A  (2x)
Keywords :  Tubr, Winged Helix-Turn-Helix, Plasmid Segregation, Dna Binding Protein (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  L. Ni, W. Xu, M. Kumaraswami, M. A. Schumacher
From The Cover: Plasmid Protein Tubr Uses A Distinct Mode O Hth-Dna Binding And Recruits The Prokaryotic Tubulin Homolo Tubz To Effect Dna Partition.
Proc. Natl. Acad. Sci. Usa V. 107 11763 2010
PubMed-ID: 20534443  |  Reference-DOI: 10.1073/PNAS.1003817107

(-) Compounds

Molecule 1 - PUTATIVE DNA-BINDING PROTEIN
    Chains: A
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PET15B(+)
    Expression System Strain: BL21-GOLD(DE3)
    Expression System Taxid: 469008
    Expression System Vector Type: PLASMID
    Gene: PBT157
    Organism Scientific: BACILLUS THURINGIENSIS
    Organism Taxid: 1430
    Strain: SEROVAR ISRAELENSIS

 Structural Features

(-) Chains, Units

  1
Asymmetric Unit : A
Biological Unit 1 (2x): A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (0, 0)

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 Sequence-Structure Mapping

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(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
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  UniProt sequence: complete  aligned part    
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Chain A from PDB  Type:PROTEIN  Length:93
 aligned with Q8KNP2_BACTI | Q8KNP2 from UniProtKB/TrEMBL  Length:104

    Alignment length:93
                                    15        25        35        45        55        65        75        85        95   
          Q8KNP2_BACTI    6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
               SCOP domains --------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ....hhhhhhhhhh.hhhhhhhhhhh..........hhhhhhhhh..hhhhhhhhhhhhhhh..eeeeee..eeeeeehhhhhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------- Transcript
                  3m9a A  6 FYTLNIAEIAERIGNDDCAYQVLMAFINENGEAQMLNKTAVAEMIQLSKPTVFATVNSFYCAGYIDETRVGRSKIYTLSDLGVEIVECFKQKA 98
                                    15        25        35        45        55        65        75        85        95
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 Classification and Annotation

(-) SCOP Domains  (0, 0)

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(-) Pfam Domains  (0, 0)

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(-) Gene Ontology  (1, 1)

Asymmetric Unit(hide GO term definitions)
Chain A   (Q8KNP2_BACTI | Q8KNP2)
molecular function
    GO:0003677    DNA binding    Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/TrEMBL
        Q8KNP2_BACTI | Q8KNP2: 3m8e 3m8f 4aso 4ass

(-) Related Entries Specified in the PDB File

3m8e