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(-) Description

Title :  AN X-RAY STRUCTURE OF A PUTATIVE PHOSPHOGYLCERATE KINASE FROM FRANCISELLA TULARENSIS SUBSP. TULARENSIS SCHU S4
 
Authors :  J. S. Brunzelle, Z. Wawrzak, T. Skarina, E. Gordon, W. F. Anderson, A. Sa Center For Structural Genomics Of Infectious Diseases (Csgi
Date :  02 Apr 12  (Deposition) - 09 May 12  (Release) - 09 May 12  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.71
Chains :  Asym. Unit :  A,B
Biol. Unit 1:  A  (1x)
Biol. Unit 2:  B  (1x)
Biol. Unit 3:  A (1x),B (1x)
Keywords :  Structural Genomics, Center For Structural Genomics Of Infectious Diseases, Csgid, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  J. S. Brunzelle, Z. Wawrzak, T. Skarina, E. Gordon, W. F. Anderson, A. Savchenko, Center For Structural Genomics Of Infectious Diseases
An X-Ray Structure Of A Putative Phosphogylcerate Kinase From Francisella Tularensis Subsp. Tularensis Schu S4
To Be Published
PubMed: search

(-) Compounds

Molecule 1 - PHOSPHOGLYCERATE KINASE
    Chains: A, B
    EC Number: 2.7.2.3
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Plasmid: PMCSG7
    Expression System Strain: BL21-CODONPLUS(DE3)-RIPL
    Expression System Taxid: 469008
    Expression System Vector Type: PLASMID
    Gene: FTT_1367C, PGK
    Organism Scientific: FRANCISELLA TULARENSIS SUBSP. TULARENSIS
    Organism Taxid: 177416
    Strain: SCHU S4/SCHU 4

 Structural Features

(-) Chains, Units

  12
Asymmetric Unit : AB
Biological Unit 1 (1x): A 
Biological Unit 2 (1x):  B
Biological Unit 3 (1x): A (1x)B (1x)

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (2, 11)

Asymmetric Unit (2, 11)
No.NameCountTypeFull Name
1MSE10Mod. Amino AcidSELENOMETHIONINE
2SO41Ligand/IonSULFATE ION
Biological Unit 1 (2, 6)
No.NameCountTypeFull Name
1MSE5Mod. Amino AcidSELENOMETHIONINE
2SO41Ligand/IonSULFATE ION
Biological Unit 2 (1, 5)
No.NameCountTypeFull Name
1MSE5Mod. Amino AcidSELENOMETHIONINE
2SO4-1Ligand/IonSULFATE ION
Biological Unit 3 (2, 6)
No.NameCountTypeFull Name
1MSE5Mod. Amino AcidSELENOMETHIONINE
2SO41Ligand/IonSULFATE ION

(-) Sites  (1, 1)

Asymmetric Unit (1, 1)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREGLU A:319 , ALA B:274 , ILE B:276BINDING SITE FOR RESIDUE SO4 A 401

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 4EHJ)

(-) Cis Peptide Bonds  (2, 2)

Asymmetric Unit
No.Residues
1Lys A:183 -Pro A:184
2Lys B:183 -Pro B:184

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 4EHJ)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 4EHJ)

(-) Exons   (0, 0)

(no "Exon" information available for 4EHJ)

(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:390
                                                                                                                                                                                                                                                                                                                                                                                                                                      
               SCOP domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ CATH domains
               Pfam domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Pfam domains
         Sec.struct. author ...hhhhh.....eeeee......ee..ee..hhhhhhhhhhhhhhhhh..eeeee...........hhhhhhhhhhhhhhhhhh..eeee.............eeee.hhhhh......hhhhhhhhhhh..eeee.hhhhh.......hhhhhhh.eeeehhhhhhhhhhhhhhhh.....eeeeee..hhhhhhhhhhhhh....eeeeehhhhhhhhhhh.........hhhhhhhhhhhhhhhhhhh.......eeeee.........eeee.hhh....eeeeehhhhhhhhhhhhhhh.eeeee.......hhhhhhhhhhhhhhhhhh..eeee.hhhhhhhhhhhhhhhhh.ee...hhhhhhhhh...hhhhhhhhhh.. Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ SAPs(SNPs)
                    PROSITE ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Transcript
                 4ehj A   2 SFLTLKDVDLKDKKVLVRVDFNVPVKDGKVTSKVRIEAAIPTIQYILDQGGAVILmSHLGRPTEGEYDSQFSLEPVAKALSEIINKPVKFAKDWLDGVDVKAGEIVmCENVRFNSGEKKSTDDLSKKIASLGDVFVmDAFATAHRAQASTYGVAKYIPVACAGILLTNEIQALEKALKSPKKPmAAIVGGSKVSTKLSVLNNLLDKVEILIVGGGIANTFIKAEGFDVGNSLYEQDLVAEATEILAKAKALGVNIPVPVDVRVAKEFSENAQAIIKKVSDVVADEmILDIGPESQKIIAELLKSANTILWNGPVGVFEFDNFAEGTKALSLAIAQSHAFSVAGGGDTIAAIEKFGIKDQVSYISTAGGAFLEFLEGKKLPAIEILKEKAI 391
                                    11        21        31        41        51     |  61        71        81        91       101      |111       121       131      |141       151       161       171       181   |   191       201       211       221       231       241       251       261       271       281     | 291       301       311       321       331       341       351       361       371       381       391
                                                                                  57-MSE                                            108-MSE                       138-MSE                                        185-MSE                                                                                               287-MSE                                                                                                    


Chain B from PDB  Type:PROTEIN  Length:390
                                                                                                                                                                                                                                                                                                                                                                                                                                      
               SCOP domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ SCOP domains
               CATH domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ CATH domains
               Pfam domains ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Pfam domains
         Sec.struct. author ...hhhhh.....eeeee..............hhhhhhhhhhhhhhhhhh.eeeee...........hhhhhhhhhhhhhhhhhh..eeee.............eeee.hhhhh......hhhhhhhhhhh..eeee.hhhhh.......hhhhhhh.eeeehhhhhhhhhhhhhhhh.....eeeeee..hhhhhhhhhhhhh....eeeeehhhhhhhhhhh.........hhhhhhhhhhhhhhhhhhh.......eeeee.........eeee........eeeeehhhhhhhhhhhhhhh.eeeee.......hhhhhhhhhhhhhhhhhh..eeee.hhhhhhhhhhhhhhhhh.ee...hhhhhhhhh...hhhhhhhhhhhh Sec.struct. author
                 SAPs(SNPs) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ SAPs(SNPs)
                    PROSITE ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ PROSITE
                 Transcript ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Transcript
                 4ehj B   2 SFLTLKDVDLKDKKVLVRVDFNVPVKDGKVTSKVRIEAAIPTIQYILDQGGAVILmSHLGRPTEGEYDSQFSLEPVAKALSEIINKPVKFAKDWLDGVDVKAGEIVmCENVRFNSGEKKSTDDLSKKIASLGDVFVmDAFATAHRAQASTYGVAKYIPVACAGILLTNEIQALEKALKSPKKPmAAIVGGSKVSTKLSVLNNLLDKVEILIVGGGIANTFIKAEGFDVGNSLYEQDLVAEATEILAKAKALGVNIPVPVDVRVAKEFSENAQAIIKKVSDVVADEmILDIGPESQKIIAELLKSANTILWNGPVGVFEFDNFAEGTKALSLAIAQSHAFSVAGGGDTIAAIEKFGIKDQVSYISTAGGAFLEFLEGKKLPAIEILKEKAI 391
                                    11        21        31        41        51     |  61        71        81        91       101      |111       121       131      |141       151       161       171       181   |   191       201       211       221       231       241       251       261       271       281     | 291       301       311       321       331       341       351       361       371       381       391
                                                                                  57-MSE                                            108-MSE                       138-MSE                                        185-MSE                                                                                               287-MSE
   Legend:   → Mismatch (orange background)
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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 4EHJ)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 4EHJ)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 4EHJ)

(-) Gene Ontology  (8, 8)

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  2.7.2.3
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        PGK_FRATT | Q5NF76: 4fey

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