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(-) Description

Title :  CHIMERIC GST
 
Authors :  A. Axarli, A. W. Muleta, E. G. Chronopoulou, A. C. Papageorgiou, N. E. La
Date :  23 Jun 16  (Deposition) - 21 Sep 16  (Release) - 21 Dec 16  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  2.33
Chains :  Asym./Biol. Unit :  A,B
Keywords :  Directed Evolution, Glutathione Transferase A1-1, Protein Stability, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  I. Axarli, A. W. Muleta, E. G. Chronopoulou, A. C. Papageorgiou, N. E. Labrou
Directed Evolution Of Glutathione Transferases Towards A Selective Glutathione-Binding Site And Improved Oxidative Stability.
Biochim. Biophys. Acta V. 1861 3416 2017
PubMed-ID: 27612661  |  Reference-DOI: 10.1016/J.BBAGEN.2016.09.004

(-) Compounds

Molecule 1 - GLUTATHIONE S-TRANSFERASE A1,GLUTATHIONE S-TRANSFERASE ALPHA-2,GLUTATHIONE S-TRANSFERASE A1,GLUTATHIONE S-TRANSFERASE ALPHA- 2,GLUTATHIONE S-TRANSFERASE A1
    Chains: A, B
    EC Number: 2.5.1.18, 2.5.1.18, 2.5.1.18, 2.5.1.18, 2.5.1.18
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Taxid: 562
    Fragment: UNP RESIDUES 1-54,UNP RESIDUES 54-65,UNP RESIDUES 66-85,UNP RESIDUES 86-213,UNP RESIDUES 214-222
    Gene: GSTA1, GSTA2
    Organism Common: HUMAN, NORWAY RAT
    Organism Scientific: HOMO SAPIENS, RATTUS NORVEGICUS
    Organism Taxid: 9606, 10116
    Other Details: PROTEIN OBTAINED AFTER GENE SHUFFLING OF HUMAN AND RAT GSTA1-1
    Synonym: GST HA SUBUNIT 1,GST CLASS-ALPHA MEMBER 1,GST-EPSILON,GSTA1- 1,GTH1,GST 1B-1B,GST A2-2,GLUTATHIONE S-TRANSFERASE YA-2,GST YA2,GST HA SUBUNIT 1,GST CLASS-ALPHA MEMBER 1,GST-EPSILON,GSTA1-1,GTH1,GST 1B-1B,GST A2-2,GLUTATHIONE S-TRANSFERASE YA-2,GST YA2,GST HA SUBUNIT 1,GST CLASS-ALPHA MEMBER 1,GST-EPSILON,GSTA1-1,GTH1

 Structural Features

(-) Chains, Units

  12
Asymmetric/Biological Unit : AB

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 1)

Asymmetric/Biological Unit (1, 1)
No.NameCountTypeFull Name
1GSH1Ligand/IonGLUTATHIONE

(-) Sites  (1, 1)

Asymmetric Unit (1, 1)
No.NameEvidenceResiduesDescription
1AC1SOFTWARETYR A:9 , ARG A:15 , ARG A:45 , GLN A:54 , VAL A:55 , PRO A:56 , GLN A:67 , THR A:68 , HOH A:422 , HOH A:428 , HOH A:460 , ASP B:101 , ARG B:131binding site for residue GSH A 301

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 5LCZ)

(-) Cis Peptide Bonds  (2, 2)

Asymmetric/Biological Unit
No.Residues
1Val A:55 -Pro A:56
2Val B:55 -Pro B:56

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5LCZ)

(-) PROSITE Motifs  (0, 0)

(no "PROSITE Motif" information available for 5LCZ)

(-) Exons   (0, 0)

(no "Exon" information available for 5LCZ)

(-) Sequences/Alignments

Asymmetric/Biological Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
  Number of residues per labelling interval =   
  UniProt sequence: complete  aligned part    
   Show mapping: SCOP domains CATH domains Pfam domains Secondary structure (by author)
SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:195
                                                                                                                                                                                                                                   
               SCOP domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ....eeeee..hhhhhhhhhhhhhh....eeeee.hhhhhhhhhhh.........eeee..eeeehhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhh...hhhhhhhhhhhhhhhhhhhhhh.........hhhhhhhhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhhh........... Sec.struct. author
                 SAPs(SNPs) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5lcz A   2 AEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDMKERALIDMYSEGILDLTEMITALAKDRTKNRYLPAFEKVLKSHGQDYLVGNRLTRVDIHLLELLLYVEEFDASLLTSFPLLKAFKSRISSLPNVKKFLQPGSQRKPAMDA 210
                                    11        21        31        41        51        61        71        81        91       101    || 125       135       145       155       165       175       185       195       205     
                                                                                                                                  106|                                                                                         
                                                                                                                                   121                                                                                         


Chain B from PDB  Type:PROTEIN  Length:209
                                                                                                                                                                                                                                                 
               SCOP domains ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ....eeee...hhhhhhhhhhhhhh....eeee..hhhhhhhhhhh.........eeee..eeeehhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhhhhh....hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh.........hhhhhhhhhhhhhhhhhhhhhhh.hhhhhhhhhhhhhhhhhhhhhh........... Sec.struct. author
                 SAPs(SNPs) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5lcz B   2 AEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDMKERALIDMYSEGILDLTEMIIQLVICPPDQREAKTALAKDRTKNRYLPAFEKVLKSHGQDYLVGNRLTRVDIHLLELLLYVEEFDASLLTSFPLLKAFKSRISSLPNVKKFLQPGSQRKPAMDA 210
                                    11        21        31        41        51        61        71        81        91       101       111       121       131       141       151       161       171       181       191       201
   Legend:   → Mismatch (orange background)
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  x → Chemical Group (purple background, 'x', labelled with number + name, e.g. ACE or NH2)
  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5LCZ)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5LCZ)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5LCZ)

(-) Gene Ontology  (15, 20)

Asymmetric/Biological Unit(hide GO term definitions)

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        GSTA1_HUMAN | P08263: 1gsd 1gse 1gsf 1guh 1k3l 1k3o 1k3y 1lbk 1pkw 1pkz 1pl1 1pl2 1usb 1xwg 1ydk 2r3x 2r6k 3i69 3i6a 3ik9 3ktl 3l0h 3q74 3u6v 3zfb 3zfl 4hj2 5jcu 5ld0
        GSTA2_RAT | P04903: 5ld0

(-) Related Entries Specified in the PDB File

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