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(-) Description

Title :  CHIMERIC GST
 
Authors :  A. Axarli, A. W. Muleta, E. G. Chronopoulou, A. C. Papageorgiou, N. E. La
Date :  23 Jun 16  (Deposition) - 21 Sep 16  (Release) - 21 Dec 16  (Revision)
Method :  X-RAY DIFFRACTION
Resolution :  1.60
Chains :  Asym. Unit :  A
Biol. Unit 1:  A  (2x)
Keywords :  Directed Evolution, Glutathione Transferase A1-1, Protein Stability, Transferase (Keyword Search: [Gene Ontology, PubMed, Web (Google)] )
 
Reference :  I. Axarli, A. W. Muleta, E. G. Chronopoulou, A. C. Papageorgiou, N. E. Labrou
Directed Evolution Of Glutathione Transferases Towards A Selective Glutathione-Binding Site And Improved Oxidative Stability.
Biochim. Biophys. Acta V. 1861 3416 2017
PubMed-ID: 27612661  |  Reference-DOI: 10.1016/J.BBAGEN.2016.09.004

(-) Compounds

Molecule 1 - GLUTATHIONE S-TRANSFERASE A1,GLUTATHIONE S-TRANSFERASE ALPHA-2,GLUTATHIONE S-TRANSFERASE A1
    Chains: A
    EC Number: 2.5.1.18, 2.5.1.18, 2.5.1.18
    Engineered: YES
    Expression System: ESCHERICHIA COLI
    Expression System Taxid: 562
    Fragment: UNP RESIDUES 1-85,UNP RESIDUES 86-213,UNP RESIDUES 214-222
    Gene: GSTA1, GSTA2
    Organism Common: HUMAN, NORWAY RAT
    Organism Scientific: HOMO SAPIENS, RATTUS NORVEGICUS
    Organism Taxid: 9606, 10116
    Other Details: CHIMERIC PROTEIN FROM GENE SHUFFLING OF RAT AND HUMAN GSTA1-1
    Synonym: GST HA SUBUNIT 1,GST CLASS-ALPHA MEMBER 1,GST-EPSILON,GSTA1- 1,GTH1,GST 1B-1B,GST A2-2,GLUTATHIONE S-TRANSFERASE YA-2,GST YA2,GST HA SUBUNIT 1,GST CLASS-ALPHA MEMBER 1,GST-EPSILON,GSTA1-1,GTH1

 Structural Features

(-) Chains, Units

  1
Asymmetric Unit : A
Biological Unit 1 (2x): A

Summary Information (see also Sequences/Alignments below)

(-) Ligands, Modified Residues, Ions  (1, 1)

Asymmetric Unit (1, 1)
No.NameCountTypeFull Name
1CL1Ligand/IonCHLORIDE ION
Biological Unit 1 (0, 0)
No.NameCountTypeFull Name
1CL-1Ligand/IonCHLORIDE ION

(-) Sites  (1, 1)

Asymmetric Unit (1, 1)
No.NameEvidenceResiduesDescription
1AC1SOFTWAREASN A:73 , HOH A:454binding site for residue CL A 301

(-) SS Bonds  (0, 0)

(no "SS Bond" information available for 5LD0)

(-) Cis Peptide Bonds  (1, 1)

Asymmetric Unit
No.Residues
1Val A:55 -Pro A:56

 Sequence-Structure Mapping

(-) SAPs(SNPs)/Variants  (0, 0)

(no "SAP(SNP)/Variant" information available for 5LD0)

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(-) Exons   (0, 0)

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(-) Sequences/Alignments

Asymmetric Unit
   Reformat: Number of residues per line =  ('0' or empty: single-line sequence representation)
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SAPs(SNPs) PROSITE motifs Exons
(details for a mapped element are shown in a popup box when the mouse pointer rests over it) 
Chain A from PDB  Type:PROTEIN  Length:206
                                                                                                                                                                                                                                              
               SCOP domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SCOP domains
               CATH domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CATH domains
               Pfam domains -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Pfam domains
         Sec.struct. author ....eeee...hhhhhhhhhhhhhh....eeee..hhhhhhhhhhh.........eeee..eeeehhhhhhhhhhhhh.....hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh.........hhhhhhhhhhhhhhhhhhhhhhh.hhhhhhhhhhhhhhhhhhhhhh........ Sec.struct. author
                 SAPs(SNPs) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- SAPs(SNPs)
                    PROSITE -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- PROSITE
                 Transcript -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Transcript
                 5ld0 A   2 AEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDMKERALIDMYSEGILDLTEMIIQLVICPPDQREAKTALAKDRTKNRYLPAFEKVLKSHGQDYLVGNRLTRVDIHLLELLLYVEEFDASLLTSFPLLKAFKSRISSLPNVKKFLQPGSQRKPA 207
                                    11        21        31        41        51        61        71        81        91       101       111       121       131       141       151       161       171       181       191       201
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  extra numbering lines below/above indicate numbering irregularities and modified residue names etc., number ends below/above '|'

 Classification and Annotation

(-) SCOP Domains  (0, 0)

(no "SCOP Domain" information available for 5LD0)

(-) CATH Domains  (0, 0)

(no "CATH Domain" information available for 5LD0)

(-) Pfam Domains  (0, 0)

(no "Pfam Domain" information available for 5LD0)

(-) Gene Ontology  (15, 20)

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 Related Entries

(-) Entries Sharing at Least One Protein Chain (UniProt ID)

UniProtKB/Swiss-Prot
        GSTA1_HUMAN | P08263: 1gsd 1gse 1gsf 1guh 1k3l 1k3o 1k3y 1lbk 1pkw 1pkz 1pl1 1pl2 1usb 1xwg 1ydk 2r3x 2r6k 3i69 3i6a 3ik9 3ktl 3l0h 3q74 3u6v 3zfb 3zfl 4hj2 5jcu 5lcz
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